切换至 "中华医学电子期刊资源库"

中华神经创伤外科电子杂志 ›› 2021, Vol. 07 ›› Issue (01) : 12 -16. doi: 10.3877/cma.j.issn.2095-9141.2021.01.003

所属专题: 文献

颅脑损伤

异甘草素对颅脑损伤大鼠IL-6和IL-10表达的影响
孙建军1, 周杰1,(), 李长栋1, 荔志云1   
  1. 1. 730050 兰州,联勤保障部队第九四〇医院神经外科
  • 收稿日期:2021-01-06 出版日期:2021-02-15
  • 通信作者: 周杰
  • 基金资助:
    甘肃省自然科学基金(1506RJZA301)

Effect of isoliquiritigenin on the expression of IL-6 and IL-10 factor in rats after traumatic brain injury

Jianjun Sun1, Jie Zhou1,(), Changdong Li1, Zhiyun Li1   

  1. 1. Department of Neurosurgery, The 940th Hospital of Joint Logistic Support Force of Chinese People’s Liberation Army, Lanzhou 730050, China
  • Received:2021-01-06 Published:2021-02-15
  • Corresponding author: Jie Zhou
引用本文:

孙建军, 周杰, 李长栋, 荔志云. 异甘草素对颅脑损伤大鼠IL-6和IL-10表达的影响[J]. 中华神经创伤外科电子杂志, 2021, 07(01): 12-16.

Jianjun Sun, Jie Zhou, Changdong Li, Zhiyun Li. Effect of isoliquiritigenin on the expression of IL-6 and IL-10 factor in rats after traumatic brain injury[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2021, 07(01): 12-16.

目的

探讨异甘草素(ISL)对大鼠颅脑创伤(TBI)后炎性因子白介素-6(IL-6)和抗炎因子IL-10表达的影响及TBI后脑保护作用。

方法

Feeney法将75只雄性Wistar大鼠制作TBI模型,按随机数字表法分为空白对照组(5只)、TBI组(35只)及ISL干预组(35只)。酶联免疫吸附试验法分别测定TBI后以及TBI经ISL干预后3、12、24、48、72 h及5、7 d各时间点IL-6及IL-10的变化,同时用干湿重法测量各时间点脑组织含水量变化,找出其相关性及内在联系。

结果

3组大鼠的IL-6及IL-10表达水平比较,差异均有统计学意义(P<0.05),经各时间点测定比较,IL-6及IL-10在TBI组及ISL干预组均较对照组均有所上升。TBI经ISL干预后,ISL干预组IL-6的表达水平在各个时间点较TBI组下降,IL-10的表达水平在各个时间点较TBI组升高,差异均有统计学意义(P<0.05)。TBI后脑组织含水量有所增加,经ISL干预后脑组织含水量在72 h及5 d下降明显,差异有统计学意义(P<0.05)。经Pearson相关分析,TBI组随着IL-6的升高,IL-10亦相应升高,呈正相关(r=0.925,P=0.000);ISL干预组IL-6升高而IL-10升高,呈正相关(r=0.888,P=0.000)。

结论

ISL可通过抑制TBI后炎性因子过度表达及促进抗炎因子表达水平,减轻TBI后炎性反应,减轻脑水肿而发挥脑保护作用。

Objective

To investigate the effect of isoliquiritigenin (ISL) on the expression of inflammatory factor interleukin-6 (IL-6) and anti-inflammatory factor IL-10 after traumatic brain injury (TBI) in rats, and the protective effect of ISL on brain after TBI.

Methods

Seventy five male Wistar rats were randomly divided into blank control group (n=5), TBI group (n=35) and ISL intervention group (n=35) according to the random table, after making TBI model by Feeney method. The enzyme-linked immunosorbent assay method was used to measure the changes of IL-6 and IL-10 at each time point of 3, 12, 24, 48, 72 h, 5 and 7 d after TBI and after TBI intervention by ISL, and colleagues used dry and wet weight method to measure the changes in brain tissue water content, find out its correlation and internal connections.

Results

The expression levels of IL-6 and IL-10 in TBI group and ISL intervention group were significantly higher than those in control group (P<0.05). After intervention with ISL, the expression level of IL-6 in TBI group was lower than that in TBI group at each time point, while the expression level of IL-10 in TBI group was higher than that in TBI group at each time point, and the differences were statistically significant (P<0.05). The water content of brain tissue increased after TBI, and decreased significantly at 72 h and 5 d after ISL intervention (P<0.05). Pearson correlation analysis showed that with the increase of IL-6, the level of IL-10 in TBI group was also increased, there is a positive correlation between them (r=0.925, P=0.000); IL-6 was increased and IL-10 was increased in ISL intervention group, there is a positive correlation between them(r=0.888, P=0.000).

Conclusion

ISL can protect the brain by inhibiting the over expression of inflammatory factors and promoting the expression of anti-inflammatory factors after TBI, reducing the inflammatory reaction and brain edema after TBI.

图1 3组各时间位点IL-6及IL-10表达水平比较
图2 TBI组与ISL干预组的IL-6和IL-10相关性分析
图3 3组各时间位点脑组织含水量比较
[1]
Brahmajothi MV, Abou-Donia MB. Monitoring from battlefield to bedside: serum repositories help identify biomarkers, perspectives on mild traumatic brain injury[J]. Mil Med, 2020, 185(Suppl 1): 197-204.
[2]
Di Battista AP, Rhind SG, Richards D, et al. An investigation of plasma interleukin-6 in sport-related concussion[J]. PLoS One, 2020, 15(4): e0232053.
[3]
Lagerstedt L, Egea-Guerrero JJ, Rodríguez-Rodríguez A, et al. Early measurement of interleukin-10 predicts the absence of CT scan lesions in mild traumatic brain injury[J]. PLoS One, 2018, 13(2): e0193278.
[4]
孙建军,李长栋,荔志云.异甘草素的脑保护作用研究进展[J].现代生物医学进展, 2012, 12(14): 2791-2793.
[5]
郭异之,蒋宇钢,刘军.血清HIF-1α、IL-6对重型颅脑损伤患者近期预后的预测价值[J].中国神经精神疾病杂志, 2019, 45(6): 365-369.
[6]
孙建军,李长栋,杨平,等.中、重型颅脑损伤病人血糖、血肌酐的变化对疾病转归的影响[J].中国临床神经外科杂志, 2020, 25(1): 1-3.
[7]
甄志勇,郭丽娟,薛增珍,等.重症颅脑损伤病人炎症反应与凝血机制特点分析[J].中西医结合心脑血管病杂志, 2020, 18(18): 3100-3104.
[8]
齐亚南,潘树义,郭大志.早期不同压力高压氧对中重型颅脑创伤大鼠的神经功能及IL-1β、IL-10和SOD表达的影响[J].转化医学杂志, 2020, 9(6): 321-325.
[9]
李晋,赵中平,蔡奕纯,等. IL-6、Pro-BNP以及hs-CRP在颅脑损伤患者脑脊液中的表达及意义[J].中国医药科学, 2017, 7(3): 113-115, 141.
[10]
Edwards KA, Gill JM, Pattinson CL, et al. Interleukin-6 is associated with acute concussion in military combat personnel[J]. BMC Neurol, 2020, 20(1): 209.
[11]
Lafrenaye AD, Mondello S, Wang KK, et al. Circulating GFAP and Iba-1 levels are associated with pathophysiological sequelae in the thalamus in a pig model of mild TBI [J]. Sci Rep, 2020, 10(1): 13369.
[12]
Armstead WM, Vavilala MS. Propranolol protects cerebral autoregulation and reduces hippocampal neuronal cell death through inhibition of interleukin-6 upregulation after traumatic brain injury in pigs[J]. Br J Anaesth, 2019, 123(5): 610-617.
[13]
Caglayan B, Kilic E, Dalay A, et al. Allyl isothiocyanate attenuates oxidative stress and inflammation by modulating Nrf2/HO-1 and NF-κB pathways in traumatic brain injury in mice [J]. Mol Biol Rep, 2019, 46(1): 241-250.
[14]
Trautz F, Franke H, Bohnert S, et al. Survival-time dependent increase in neuronal IL-6 and astroglial GFAP expression in fatally injured human brain tissue[J]. Sci Rep, 2019, 9(1): 11771.
[15]
Peltz CB, Kenney K, Gill J, et al. Blood biomarkers of traumatic brain injury and cognitive impairment in older veterans[J]. Neurology, 2020, 95(9): e1126-e1133.
[16]
Aisiku IP, Yamal JM, Doshi P, et al. Plasma cytokines IL-6, IL-8, and IL-10 are associated with the development of acute respiratory distress syndrome in patients with severe traumatic brain injury[J]. Crit Care, 2016, 20: 288.
[17]
Deng Y, Jiang X, Deng X, et al. Pioglitazone ameliorates neuronal damage after traumatic brain injury via the PPARγ/NF-κB/IL-6 signaling pathway[J]. Genes Dis, 2020, 7(2): 253-265.
[18]
Posti JP, Takala R, Lagerstedt L, et al. Correlation of blood biomarkers and biomarker panels with traumatic findings on computed tomography after traumatic brain injury[J]. J Neurotrauma, 2019, 36(14): 2178-2189.
[19]
杨永明,荔志云,季玮.异甘草素对创伤性脑损伤大鼠血清细胞因子的影响[J].神经损伤与功能重建, 2014, 9(2): 108-110.
[20]
杨永明,荔志云.异甘草素对创伤性脑损伤大鼠损伤灶修复作用的实验研究[J].创伤外科杂志, 2014, 16(2): 144-146.
[21]
季玮,李长栋,荔志云,等.异甘草素对PC12细胞缺氧前后LDH和SOD活性的影响[J].中华神经外科疾病研究杂志, 2016, 15(2): 132-135.
[1] 刘立, 陈诚, 李新科, 刘凯, 屠昌明. 血清IL-6、hs-CRP、MMP-9联合检测在腹股沟疝无张力修补术预后评价中的价值分析[J]. 中华疝和腹壁外科杂志(电子版), 2023, 17(04): 405-409.
[2] 钱晓英, 吴新, 徐婷婷. 颅脑损伤并发呼吸衰竭患者早期机械通气的效果分析[J]. 中华肺部疾病杂志(电子版), 2023, 16(04): 526-528.
[3] 刘玲, 肖颖, 王蓉. 严重创伤并发肺部感染死亡病例分析[J]. 中华肺部疾病杂志(电子版), 2023, 16(04): 581-583.
[4] 丁晓静, 张丹琦, 杨伟. 布地奈德雾化吸入联合金嗓开音胶囊治疗小儿急性喉炎的疗效及对IFN-γ、SAA、IL-6的影响[J]. 中华肺部疾病杂志(电子版), 2023, 16(03): 391-393.
[5] 李飞翔, 段虎斌, 李晋虎, 吴昊, 王永红, 范益民. 急性颅脑损伤继发下肢静脉血栓的相关危险因素分析及预测模型构建[J]. 中华神经创伤外科电子杂志, 2023, 09(05): 277-282.
[6] 潘立, 谢理政, 程宏伟, 茆翔. 创伤性颅脑损伤后垂体功能减退[J]. 中华神经创伤外科电子杂志, 2023, 09(05): 308-312.
[7] 王景景, 符锋, 李建伟, 任党利, 陈翀, 刘慧, 孙洪涛, 涂悦. 针刺对中型创伤性颅脑损伤后BDNF/TrkB信号通路的影响[J]. 中华神经创伤外科电子杂志, 2023, 09(04): 199-205.
[8] 张馨月, 韩帅, 张舒石, 李文臣, 张舒岩. 颅内压监测技术在创伤性颅脑损伤治疗中的应用[J]. 中华神经创伤外科电子杂志, 2023, 09(04): 246-252.
[9] 王召, 田进杰, 郭朝, 王蕾, 严红燕, 冯素娟, 张毅. 血浆PGK1早期检测对创伤性颅脑损伤患者病情严重程度及预后的预测价值[J]. 中华神经创伤外科电子杂志, 2023, 09(03): 154-159.
[10] 何佳伟, 张良, 杨骐, 王占祥. 创伤性颅脑损伤后进展性出血性损伤的诊疗现状[J]. 中华神经创伤外科电子杂志, 2023, 09(03): 175-179.
[11] 贾素英, 李倩, 郭姗姗. 创伤性颅脑损伤后血小板功能障碍的研究进展[J]. 中华神经创伤外科电子杂志, 2023, 09(03): 180-185.
[12] 张永明. 颈段脊髓电刺激治疗颅脑损伤后慢性意识障碍的进展[J]. 中华神经创伤外科电子杂志, 2023, 09(03): 129-134.
[13] 王守森, 黄银兴, 陈宇晖, 胡晓芳, 刘海兵. 重型颅脑损伤的外科救治策略[J]. 中华神经创伤外科电子杂志, 2023, 09(03): 190-192.
[14] 汤宏, 高灵, 高宁, 蒋俊文, 张吉坤, 李由, 陈伟明, 夏鹰. 控制性减压在治疗重型颅脑损伤中的应用[J]. 中华神经创伤外科电子杂志, 2023, 09(02): 91-96.
[15] 丁晶, 李培雯, 许迎春. 醒脑开窍针刺法在神经急重症中的应用[J]. 中华针灸电子杂志, 2023, 12(04): 161-164.
阅读次数
全文


摘要