Effects of Artesunate on gene Claudin-5 expression which could influent cell mechanical properties in Glioma

doi:10.3877/cma.j.issn.2095-9141.2017.06.009

Abstract

Abstract:

Objective

To observe the effects of Artesunate (Art) on expression of gene Claudin-5 in Glioma and cell mechaniacal properties by different biological detecting methods.

Methods

After treated by Art, the expression of gene Claudin-5 in SHG44 had been detected by polymerase chain reaction method; cell migration had been detected by trans-well method. And we used atomic force microscope to observe the change of cell mechanical properties.

Results

The Art could upregulate the expression of gene Claudin-5, which is the intercellular tight junction protein, leading to change of the mechanical properties of SHG44 cells to inhibit SHG44 cell migration effectively. The cell adhesive force increased significantly after 30 mg/l Art treatment (2600 ± 400) pN vs (3800 ± 300) pN. And the cell elasticity decreased significantly after 30 mg/l Art treatment (26 ± 6) MPa vs (4.7 ± 1.6) MPa. The membrane surface roughness of SHG44 increased significantly (0.15 ± 0.013) μm vs (0.33 ± 0.022) μm after 30 mg/l Art treatment.

Conclusion

The Art, which could be a potential anti-tumor drug, could affect the biological characteristics of SHG44 through regulating the expression of gene Claudin-5 to resist Glioma.

Key words: Artesunate, Glioma, Gene Claudin-5, Cell mechanical properties.

Shizhong Lian, Jing Feng, Wang Miao, Geng Guo, Hongqin Wang, Xiaoling Hu, Bin Song, Yongming Xu, Ming Xu, Xun Huang, Ruyi Shi. Effects of Artesunate on gene Claudin-5 expression which could influent cell mechanical properties in Glioma[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2017, 03(06): 360-364.

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Figures/Tables 5

References 16

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基因名称	引物序列（5 ′ -3 ′）	产物大小(bp)	退火温度（℃）
GaPDH ^[2]	Forward: TTCGTACCTGGCATTGACTGG	?	?
GaPDH ^[2]	Reverse: GAAGGTGAAGGTCGGAGT	225	57
Claudin-5 ^[2]	Forward: TTCGCCAACATTGTCGTCC	?	?
Claudin-5 ^[2]	Reverse: TCTTCTTGTCGTAGTCGCCG	232	63

基因名称	引物序列（5 ′ -3 ′）	产物大小(bp)	退火温度（℃）
GaPDH ^[2]	Forward: TTCGTACCTGGCATTGACTGG	?	?
GaPDH ^[2]	Reverse: GAAGGTGAAGGTCGGAGT	225	57
Claudin-5 ^[2]	Forward: TTCGCCAACATTGTCGTCC	?	?
Claudin-5 ^[2]	Reverse: TCTTCTTGTCGTAGTCGCCG	232	63

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