Silencing GLI1 by siRNA inhibits the proliferation of glioma U87 cells

doi:10.3877/cma.j.issn.2095-9141.2015.05.008

Abstract

Abstract:

Objective

To investigate the effect of glioma associated oncogene 1 (GLI1) reduction by small interference RNAs (siRNA) on cell proliferation and glucose metabolism of U87 glioma cells.

Methods

siRNA for GLI1 was transfected into U87 glioma cells to inhibit the expression of GLI1. The non-transfected cells and cells transfected with non-sense siRNA were used as controls.The proliferation of U87 cells was assessed by MTT assay, and the cell cycle stage was detected by flow cytometry.

Results

The results of Western blotting showed that GLI1’s expression was significantly down-regulated in GLI1 siRNA group compared to the two control groups(P<0.05); cell proliferation was assessed by MTT assays, cell proliferation was significantly inhibited 3 days after treatment of GLI1 siRNA compared with control groups, and declined with persistently along with the extension of the culture time (P<0.05); the results of flow cytometry showed that the cell cycle was blocked at G1 phase, and the number of cells in S phase declined significantly compared with control groups (P<0.05).

Conclusion

Interference of GLI1 expression can markedly inhibit the proliferation of U87 glioma cells, indicating that GLI1 can serve as a promising therapeutic target for glioma.

Key words: GLI1, siRNA, Glioma

Jianbo Ma, Kai Sun, Chong Ma, Lei Cao, Jianping Zhao, Lukui Chen. Silencing GLI1 by siRNA inhibits the proliferation of glioma U87 cells[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2015, 01(05): 30-32.

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References 15

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