Abstract:
Objective
To investigate the effects of hypoxic preconditioning on the expression of TrxR2、CHOP and the ultra-structure of the neuronal cells of rats suffered traumatic brain injury.
Methods
Forty eight Sprague Dawley rats were randomly divided into control group (Con), hypoxic preconditioning group (HPC), traumatic brain injury group (TBI) and hypoxic preconditioning traumatic brain injury group (HPCT). The traumatic brain injury model of rats were made by the Feeney’s improved equipment and hypoxic preconditioning model were made by Hypobaric chamber for successive 3 days (-50 kPa、3 h/d). Staining HE and making ultra-thin slicing by fresh specimen to observe the potential change of general structure and ultra-structure of rats’ brain tissue of each group respectively under light microscope and electron microscope.Expressions of TrxR2、CHOP mRNA and proteins were detected in the brain cortex around the contusion area by qRT-PCR and Western blotting.
Results
The pathologic change has no difference between Con gourp and HPC group. HPCT group has a lighter pathologic change than the TBI group.The expressions of TrxR2 mRNA and protein was significantly higher in the brain cortex in the HPCT group than the TBI group, the difference between the two group was significant(P＜0.05),the expressions of CHOP mRNA and protein was significantly lower in the brain cortex in the HPCT group than the TBI group, the difference between the two group was significant (P＜0.05), while the difference between the Con group and the HPC group was not significant (P>0.05).
Conclusion
Hypoxia preconditioning can obviously reduce the craniocerebral trauma rats cortex neuron cell morphology change,which has a close relationship with the upregulated expression of TrxR2 and the downregulated expression of CHOP,in order to protection of neurons.
Key words:
Traumatic brain injury,
Hypoxic preconditioning,
TrxR2,
CHOP,
Ultra-structure
Guangjie Liu, Jiachuan Liu, Jinbiao Wang, Yanyan Yang, Xing Zhang, Chunlin Wang, Hong Tang, shen Xu. Effects of hypoxic preconditioning on cortical expression of TrxR2 and CHOP of rats with craniocerebral injury and ultramicrostructural changes of neurons[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2015, 01(03): 164-168.