Protective effect of lamotrigine on mouse dorsal spinal cord injury

doi:10.3877/cma.j.issn.2095-9141.2015.03.009

Abstract

Abstract:

Objective

To investigate the effect of lamotrigine (LTG) on recovery after spinal cord injury(SCI).

Methods

A total of 80 female C57BL/6 mouses were made the model of spinal cord hemisection, and all of these were randomly divided into 3 group:SCIgroup,SCI+LTG group and SCI+0.9%saline group;mouses in SCI+LTG grouop were given interperitoneal injection of lamotrigine(25 mg/kg)for 7days,and mouses in SCI+0.9%saline group were given the same volume of 0.9%saline.Basso,Beatti,Bresnahan(BBB)scale was perfomed 1,7,14days after SCI;the number of glial cell were observed by immunofluorescence and the express of inflammatory factors were obseverd by ELISA(enzyme linked immunosorbent assay).

Results

On the 7th, 14th days after injury, the BBB scale scores in the SCI+LTG group were significantly higher than those in the SCI group and in the SCI+0.9%saline group (P＜0.05). On the 7th, 14th days after injury, less percentage of GFAP⁺ fluorenscent area and less number of Iba1⁺cells in the SCI+LTG group were distributed in the lesion area than those in the SCI group and in the SCI+0.9%saline group(P＜0.05).On the 7th day after spinal cord injury,the IL-1 and IL-10 expression in SCI+LTG group were obviously lower than those in the SCI group and in the SCI+0.9% saline group(P＜0.05).

Conclusion

Lamotrigine improve the motor function after spinal cord injury by decrease the activation of the glial cells.

Key words: Spinal cord injury, Lamotrigine, Glialcell, Inflammatory factor

Qiang Fu, Zhaotao Wang, Maoying Zhang, Ruxiang Xu. Protective effect of lamotrigine on mouse dorsal spinal cord injury[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2015, 01(03): 158-163.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhsjcswkdzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-9141.2015.03.009

https://zhsjcswkdzzz.cma-cmc.com.cn/EN/Y2015/V01/I03/158

Figures/Tables 6

References 19

[1]	邹明明,薛兴森,朱海涛,等.姜黄素促进大鼠脊髓损伤恢复的病理及行为学研究[J]. 第三军医大学学报, 2011, 33(8):765-770.
[2]	Popiolek- Barczyk K, Kolosowska N, Piotrowska A, et al.Parthenolide Relieves Pain and PromotesM2 Microglia/Macrophage Polarization in Rat Model of Neuropathy[J].Neural Plasticity,2015,2015.
[3]	Tsuda M. [Mechanisms underlying the pathogenesis of neuropathic pain revealing by the role of glial cells] [J]. Nihon shinkei seishin yakurigaku zasshi= Japanese journal of psychopharmacology,2015,35(1):1-4.
[4]	Clark AK, Gruber-Schoffnegger D, Drdla-Schutting R, et al.Selective Activation of Microglia Facilitates Synaptic Strength[J].The Journal of Neuroscience,2015,35(11):4552-4570.
[5]	Berliocchi L, Maiarù M, Varano GP, et al. Spinal autophagy is differently modulated in distinct mouse models of neuropathic pain[J].Molecular pain,2015,11(1):3.
[6]	Yin Q, Fan Q, Zhao Y, et al. Spinal NF-κB and Chemokine Ligand 5 Expression during Spinal Glial Cell Activation in a Neuropathic Pain Model[J].PloS one,2015,10(1):e0115120.
[7]	Choi YS, Jun IG, Kim SH, et al. Intrathecal Lamotrigine Attenuates Mechanical Allodynia and Suppresses Microglial and Astrocytic Activation in a Rat Model of Spinal Nerve Ligation[J].Yonsei medical journal,2013,54(2):321-329.
[8]	Dag E, Dag ZO, Baydas G, et al. Effects of lamotrigine and topiramate on brain maturation and cognitive functions in offspring of pregnant rats-preliminary study[J]. Advances in clinical and experimental medicine: official organ Wroclaw Medical University,2014,23(5):691.
[9]	Floriddia EM, Rathore KI,Tedeschi A, et al. p53 Regulates the neuronal intrinsic and extrinsic responses affecting the recovery of motor function following spinal cord injury[J]. The Journal of Neuroscience,2012,32(40):13956-13970.
[10]	Zhang MY, Zheng CY, Zou MM, et al. Lamotrigine attenuates deficits in synaptic plasticity and accumulation of amyloid plaques in APP/PS1 transgenic mice[J]. Neurobiology of aging,2014,35(12):2713-2725.
[11]	张鸿日,彭静华,张茂营,等.2ME2 对脊髓损伤大鼠运动功能及Tau 蛋白磷酸化的影响[J].中华神经医学杂志,2014,13(011):1127-1130.
[12]	Nunes AF, Amaral JD, Lo AC, et al. TUDCA, a bile acid,attenuates amyloid precursor protein processing and amyloid-β deposition in APP/PS1 mice[J]. Molecular neurobiology, 2012,45(3):440-454.
[13]	Wiffen PJ, Derry S, Moore RA. Lamotrigine for acute and chronic pain[J].The Cochrane Library,2011.
[14]	Marchand F, Perretti M, McMahon SB. Role of the immune system in chronic pain[J]. Nature Reviews Neuroscience, 2005,6(7):521-532.
[15]	Poonuru RR, Gonugunta CSR. Bimodal gastroretentive drug delivery systems of lamotrigine: Formulation and evaluation[J].Indian journal of pharmaceutical sciences,2014,76(6):476.
[16]	Kishimoto A, Goto Y, Hashimoto K. Post- traumatic Stress Disorder Symptoms in a Female Patient Following Repeated Teasing: Treatment with Gabapentin and Lamotrigine and the Possible Role of Sensitization[J]. Clinical Psychopharmacology and Neuroscience,2014,12(3):240-242.
[17]	Jun IG, Kim SH, Yoon YI, et al. Intrathecal lamotrigine attenuates antinociceptive morphine tolerance and suppresses spinal glial cell activation in morphine-tolerant rats[J]. Journal of Korean medical science,2013,28(2):300-307.
[18]	Perisic T, Zimmermann N, Kirmeier T, et al. Valproate and amitriptyline exert common and divergent influences on global and gene promoter- specific chromatin modifications in rat primary astrocytes[J]. Neuropsychopharmacology, 2010, 35(3):792-805.
[19]	Guizzo R, Paques MW, Anhezini L, et al. Neuroprotective effects of oral lamotrigine administration on rabbit retinas after pars plana vitrectomy and silicone oil injection[J]. Retina,2008,28(4):638-644.

组别	鼠数(例)	第1天	第7天	第14天
模型组	20	2.17±0.48	4.00±0.63	5.50±0.55
治疗组	20	2.33±0.52	5.17±0.41^a	6.50±0.55^a
对照组	20	2.17±0.41	3.83±0.41	5.33±0.52
F值		0.278	12.955	8.269
P值		0.761	0.001	0.004

组别	鼠数(例)	第1天	第7天	第14天
模型组	20	2.17±0.48	4.00±0.63	5.50±0.55
治疗组	20	2.33±0.52	5.17±0.41^a	6.50±0.55^a
对照组	20	2.17±0.41	3.83±0.41	5.33±0.52
F值		0.278	12.955	8.269
P值		0.761	0.001	0.004

组别	鼠数(例)	第1天	第7天	第14天
模型组	6	18.07±1.98	12.34±1.72	7.46±1.28
治疗组	6	18.42±1.91	9.87±0.96	4.79±1.02^a
对照组	6	18.40±2.37	11.89±1.70	7.53±1.70
F值		0.217	18.444	31.664
P值		0.806	0.000	0.000

组别	鼠数(例)	第1天	第7天	第14天
模型组	6	18.07±1.98	12.34±1.72	7.46±1.28
治疗组	6	18.42±1.91	9.87±0.96	4.79±1.02^a
对照组	6	18.40±2.37	11.89±1.70	7.53±1.70
F值		0.217	18.444	31.664
P值		0.806	0.000	0.000

组别	鼠数(例)	第1天	第7天	第14天
模型组	6	206.51±61.11	355.47±23.94	172.92±21.11
治疗组	6	207.81±56.03	285.68±25.90^a	152.86±12.77^a
对照组	6	205.73±59.32	379.17±52.73	178.91±21.08
F值		0.008	44.488	12.783
P值		0.992	0.000	0.000

Please choose a citation manager

Content to export