Effect of sulbactam on brain tissue injury in rats with focal cerebral ischemia-reperfusion

doi:10.3877/cma.j.issn.2095-9141.2023.06.003

Abstract

Abstract:

Objective

To explore the effect of sulbactam on brain tissue injury in rats with focal cerebral ischemia-reperfusion.

Methods

Thirty male SD rats were selected and randomly divided into a sham group, a model group and a sulbactam group, with 10 rats in each group. The right cerebral ischemia-reperfusion model was prepared by thread embolism method. Thirty minutes after cerebral ischemia, the model group was intraperitoneally injected with 10 μL normal saline, the sulbactam group was intraperitoneally injected with 10 μL sulbactam, and the sham group was not given any intervention. Longa score was used to evaluate the neurological function of rats, Image-ProExpress imaging was used to analyze cerebral infarction volume, and immunohistochemical staining was used to detect serum glial glutamate transporter-1 (GLT-1) levels. Thirty days after surgery, Morris water maze was used to test learning and memory function, and the escape latency on days 1, 2, 3, 4, and 5 was compared.

Results

The neurological function score and cerebral infarction volume in the sham group were 0, while those in the sulbactam group were significantly lower than those in the model group (P<0.05). The serum GLT-1 levels of the three groups were statistically significant (P<0.05), the serum GLT-1 levels of the sulbactam group and the model group were significantly higher than those of the sham group, while the sulbactam group was significantly lower than the model group (P<0.05). On the first day of test, the escape latency of the three groups was not statistically significant (P>0.05). On the 2^nd to 5^th day of testing, the escape latency of the three groups was statistically significant (P<0.05), the escape latency of the sulbactam group and the model group was significantly higher than that of the sham group (P<0.05), and the escape latency of the sulbactam group was significantly lower than that of the model group (P<0.05).

Conclusion

Sulbactam can effectively improve nerve function, learning and memory function, reduce cerebral infarction volume and serum GLT-1 level in rats with focal cerebral ischemia-reperfusion.

Key words: Sulbactam, Focal cerebral ischemia, Ischemia reperfusion, Neurological function, Cerebral infarction volume, Learning and memory function, Glial glutamate transporter-1

Xin Cui, Peng Zhang, Tongnan Gu, Yanzhi Zhao. Effect of sulbactam on brain tissue injury in rats with focal cerebral ischemia-reperfusion[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2023, 09(06): 333-337.

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References 15

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组别	只数	神经功能评分（分）	脑梗死容积（mm³）	GLT-1（个/mm²）
假手术组	10	0	0	20.40±1.65
模型组	10	2.50（2.00，3.00）	385.57±22.12	75.10±3.67^a
舒巴坦组	10	1.00（1.00，1.00）^b	233.47±16.66^b	54.40±3.95^ab
t/Z/F值		-3.014	17.368	720.847
P值		0.003	<0.001	<0.001

组别	只数	神经功能评分（分）	脑梗死容积（mm³）	GLT-1（个/mm²）
假手术组	10	0	0	20.40±1.65
模型组	10	2.50（2.00，3.00）	385.57±22.12	75.10±3.67^a
舒巴坦组	10	1.00（1.00，1.00）^b	233.47±16.66^b	54.40±3.95^ab
t/Z/F值		-3.014	17.368	720.847
P值		0.003	<0.001	<0.001

组别	只数	第1天	第2天	第3天	第4天	第5天
假手术组	10	52.60±7.83	33.40±7.78	21.90±3.18	9.20±2.57	8.80±2.20
模型组	10	56.90±12.48	49.60±7.86^a	39.60±10.21^a	27.80±7.86^a	23.40±6.24^a
舒巴坦组	10	53.80±11.55	41.70±6.55^ab	30.70±8.59^ab	15.60±4.67^ab	12.50±3.75^ab
t值		0.421	11.916	12.488	29.706	29.884
P值		0.650	<0.001	<0.001	<0.001	<0.001

组别	只数	第1天	第2天	第3天	第4天	第5天
假手术组	10	52.60±7.83	33.40±7.78	21.90±3.18	9.20±2.57	8.80±2.20
模型组	10	56.90±12.48	49.60±7.86^a	39.60±10.21^a	27.80±7.86^a	23.40±6.24^a
舒巴坦组	10	53.80±11.55	41.70±6.55^ab	30.70±8.59^ab	15.60±4.67^ab	12.50±3.75^ab
t值		0.421	11.916	12.488	29.706	29.884
P值		0.650	<0.001	<0.001	<0.001	<0.001

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