To test the operability and safety of the trough sheath puncture needle used in brain tissue puncture of experimental animals.

Six New Zealand rabbits were anesthetized and bilateral cerebral hemispheres were exposed by craniotomy. The left cerebral hemisphere was punctured with traditional puncture needle. The right side was punctured with a groove sheath needle, and the steps of puncturing, pulling out the puncture needle and retaining the groove sheath, and pulling out the groove sheath after catheterization were completed. The puncture passage was stained with hematoxylin after pulling out the puncture needle. After 30 min of observation, the experimental animals were killed, the brain tissue was extracted and were cut along the puncture channel as 3 μm-thick brain tissue sections. Routine HE staining was used in pathological sectionthe, and pathological and anatomical changes (brain tissue damage and hemorrhage) of the puncture channel of the brain tissue were observed under the microscope to evaluate the injury situation.

Two kinds of puncture needle can cause local cerebral contusion in the puncture channel. The manifestations were puncture passage tissue defect, brain tissue congestion and edema. Under the light microscope, the brain tissue of the puncture passage was broken, the cells arranged irregularly, the swollen cell body was enlarged and the nucleus was pyknosis, and the peripheral cell structure of the puncture passage was normal. There was no significant difference in the pathological characteristics between the two kinds of needle channels.

The operation of the puncture is simple and convenient. It is convenient to put a drainage tube through the groove sheath. There is no significant difference between the injury degree of brain tissue and the traditional puncture needle.