Loss of TLR4 impairs motor balance in mice

doi:10.3877/cma.j.issn.2095-9141.2018.06.009

Abstract

Abstract:

Objective

To analysis the effection of Toll-like receptor 4 (TLR4) on motor balance in mice.

Methods

Compared the difference of performance in motor balance related behaviors tests between TLR4^-/- mice and its littermates (TLR4^+/+), and analysis the effection of TLR4 loss on cerebellar structures.

Results

TLR4^-/- mice exhibited no difference in the footprint test (P>0.05), but a better performance in accelerating-rotarod test compared with TLR4^+/+ mice (P<0.05). In addition, TLR4^-/- mice show poor performances in Ladder rung task than their wildtype counterparts (P<0.05). The results from H&E staining revealed that cerebellar structure has no changes in TLR4^-/- mice, but the thickness of molecular layers is decreased (P<0.05).

Conclusion

Loss of TLR4 can impair motor coordination in mice.

Key words: Toll-like receptor 4, Motor balance, Molecular layer, Purkinje cell

Jianwei Zhu, Yifei Li, Zhaotao Wang, Weiqiang Jia, Chen Chen, Ji Wang, Yingjiang Gu, Ruxiang Xu. Loss of TLR4 impairs motor balance in mice[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2018, 04(06): 353-356.

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Figures/Tables 3

References 15

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平衡能力指标		野生型	TLR4^?/?	t值	P值
只数		10	11	?	?
足迹实验		?	?	?	?
?	步长（cm）	7.362±1.123	7.032±0.985	1.068	0.299
?	步宽（cm）	2.736±0.206	2.892±0.198	-0.775	0.448
?	肢体间协调（cm）	0.664±0.034	0.711±0.029	-0.368	0.724
加速转棒实验		?	?	?	?
?	停留时间（s）	172.452±10.233	208.275±18.562	-2.303	0.033
?	落下时转速（r/min）	28.325±2.328	33.913±2.672	-2.310	0.032
梯踏步实验		?	?	?	?
?	规律梯通过时间（s）	10.58±2.148	14.42±2.366	-1.507	0.148
?	落下时转速（r/min）	1.816±0.526	4.219±0.738	-2.723	0.013
?	不规律梯通过时间（s）	16.62±2.324	23.38±4.595	-1.661	0.113

平衡能力指标		野生型	TLR4^?/?	t值	P值
只数		10	11	?	?
足迹实验		?	?	?	?
?	步长（cm）	7.362±1.123	7.032±0.985	1.068	0.299
?	步宽（cm）	2.736±0.206	2.892±0.198	-0.775	0.448
?	肢体间协调（cm）	0.664±0.034	0.711±0.029	-0.368	0.724
加速转棒实验		?	?	?	?
?	停留时间（s）	172.452±10.233	208.275±18.562	-2.303	0.033
?	落下时转速（r/min）	28.325±2.328	33.913±2.672	-2.310	0.032
梯踏步实验		?	?	?	?
?	规律梯通过时间（s）	10.58±2.148	14.42±2.366	-1.507	0.148
?	落下时转速（r/min）	1.816±0.526	4.219±0.738	-2.723	0.013
?	不规律梯通过时间（s）	16.62±2.324	23.38±4.595	-1.661	0.113

形态学指标	野生型	TLR4^?/?	t值	P值
只数	4	4	?	?
小脑面积（×10⁶ μm²）	6.322±0.218	6.092±0.166	1.292	0.244
分子层厚度（μm）	141.56±6.821	122.39±11.261	2.593	0.041
颗粒层面积（×10⁶ μm²）	2.482±0.192	2.208±0.085	1.244	0.260

形态学指标	野生型	TLR4^?/?	t值	P值
只数	4	4	?	?
小脑面积（×10⁶ μm²）	6.322±0.218	6.092±0.166	1.292	0.244
分子层厚度（μm）	141.56±6.821	122.39±11.261	2.593	0.041
颗粒层面积（×10⁶ μm²）	2.482±0.192	2.208±0.085	1.244	0.260

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