MicroRNA-182 targets inhibition of TLR4 and reduces hypoxia-induced microglial inflammatory response

doi:10.3877/cma.j.issn.2095-9141.2019.03.008

Abstract

Abstract:

Objective

To study the effect of microRNA-182 (miR-182) targeting Toll-like receptor 4 (TLR4) on microglial inflammatory response.

Methods

Microglial cells were transfected with miR-182, small interfering TLR4 RNA (siTLR4) and TLR4(ORF) overexpression plasmids using transfection reagent Lipofectamine 2000. Simulating ischemia and hypoxia by OGD treatment. Detection of TLR4 as a direct target of miR-182 by luciferase reporter assay. Detection of mRNA and relative expression of protein by RT-PCR, Western blot and ELISA.

Results

miR-182 is associated with the expression of TLR4 in microglia under hypoxic conditions, TLR4 is a direct target of miR-182, and miR-182 can inhibit the expression of TLR4, and reduce the release of inflammatory factors (TNF-α, IL-6, IL-1β) from microglia

Conclusion

miR-182 inhibits hypoxia-induced microglial inflammatory response by targeting inhibition of TLR4 expression.

Key words: MicroRNA-182, Toll-like receptor 4, Microglia, Inflammatory response

Ji Wang, Hao Zhao, Xiufen Chen, Zongyu Xiao, Jianwei Zhu, Wenjin Chen, Li Zhang, Ruxiang Xu. MicroRNA-182 targets inhibition of TLR4 and reduces hypoxia-induced microglial inflammatory response[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2019, 05(03): 163-167.

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Figures/Tables 5

References 16

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