Electroacupuncture-induced cannabinoid receptor expression in repair of aortic nerve

doi:10.3877/cma.j.issn.2095-9141.2018.05.009

Abstract

Abstract:

Objective

To investigate whether electroacupuncture can promote the repair of injured optic nerve by cannabinoid receptor-mediated regulation of microglia activation.

Methods

Healthy male Beagle dogs were randomly divided into five groups: sham operation group (A), injury group (B), electroacupuncture treatment group (C), antagonist group (D), and solvent group (E). After Beagle dogs are anesthetized, group A: only the abducens nerve was exposed and separated and bound by electroacupuncture in other groups; group B: Abducens nerve injury model was made and bound by electroacupuncture in other groups; group C: Beagle dogs abducens nerve injury model was established and electroacupuncture stimulation was carried out; group D: AM630 was injected after the injury model was successful and electroacupuncture stimulation was given; group E: They were treated with group D, but before each electroacupuncture stimulation, they were given intraperitoneal injection. Western blot was used to detect the expression of cannabinoid type 1 receptor (CB1R) and CB2R in microglia of group A, B and C. Immunofluorescence was used to analyze the expression of CB2R in 5 groups.

Results

Compared with group A, Western blot test showed no significant change in the expression of CB1R protein in group C after treatment (P>0.05). At the same time, there was no significant difference in the expression of CB1R protein in the group A compared with the group B (P>0.05). The expression of CB2R protein in group C was significantly higher than that in group A after 15 min continuous electroacupuncture treatment (P<0.01), but there was no significant difference in the expression of CB2R protein in groups A and B. The number of Arginse/CD11b cells in the C group was significantly different from that of the group A (P<0.05), while the number of Arginse/CD11b cells in the group E was also statistically significant compared with that of the group A (P<0.05).

Conclusion

The CB2R in the cannabinoid receptor is mainly involved in the electroacupuncture-induced neuroprotection. Electroacupuncture can promote the repair of injured optic nerve by CB2R-mediated microglial activation.

Key words: Abducens nerve injury, Microglia, Cannabinoid receptor, Electroacupuncture

Lei Wang, Yi Zhang, Xudong Wang, Zi Ye. Electroacupuncture-induced cannabinoid receptor expression in repair of aortic nerve[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2018, 04(05): 291-296.

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References 15

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