Effects of electroacupuncture preconditioning on behavioural and peripheral blood EPCs in cerebral ischaemia-reperfusion injury rats

doi:10.3877/cma.j.issn.2095-9141.2024.02.002

Abstract

Abstract:

Objective

To observe the effects of electroacupuncture pretreatment on behavioral and peripheral blood endothelial progenitor cells (EPCs) levels in rats with cerebral ischemia-reperfusion injury (CIRI).

Methods

Forty-five adult male SD rats were randomly divided into the sham group, the model group and the electroacupuncture pretreatment group (EA group), with 15 rats in each group. Rats in the model group were restrained for 14 d before surgery, and the middle cerebral artery occlusion ischemia-reperfusion model was established by Longa thread-occluded method; rats in the sham group were treated as the same as the rats in the model group except for not inserting thread plug; rats in the EA group were pretreated with EA 14 d before surgery, others were the same as those in the model group. Cerebral blood flow was monitored on the day of modelling, and infarct volume was calculated 1 d after modelling. At different time points after modelling, the modified neurological severity score (mNSS), the open-field experiment and the Morris water maze experiment were used to detect the relevant indexes in rats, and peripheral blood was taken to determine the levels of EPCs by flow cytometry.

Results

The cerebral blood flow at each time point during the modeling process in the model group and EA group was significantly lower than that in the sham surgery group. The cerebral blood flow in the EA group was higher than that in the model group immediately after thrombus insertion and 30 min of reperfusion, and the differences were statistically significant (P<0.05). The cerebral infarction volume percentage in the model group and EA group was significantly higher than that in the sham surgery group, while the cerebral infarction volume percentage in the EA group was significantly lower than that in the model group, with statistical significance (P<0.05). The mNSS scores of the EA group at 1, 3, 7, and 21 d after modeling were lower than those of the model group, and the difference was statistically significant (P<0.05). The average speed of the open field experiment in EA group rats was significantly higher than that of the model group 1 d after modeling, and the percentage of target quadrant retention time was higher than that of the model group 3 d after modeling, the differences were statistically significant (P<0.05). The peripheral blood EPCs level was higher than that of the model group 2 d after modeling, and the differences were statistically significant (P<0.05).

Conclusion

Electroacupuncture pretreatment can improve the cerebral blood flow status of acute phase CIRI rats to a certain extent, alleviate neurological damage, enhance learning and memory abilities and motor abilities, and increase the level of EPCs in peripheral blood.

Key words: Electroacupuncture pretreatment, Cerebral ischemia-reperfusion injury, Middle cerebral artery embolism, Endothelial progenitor cells

Yi Zhang, Xiujun Ren, Mengwei Guo, Yafang Zhao, Yifan Li, Jiayang Li, Xiaoxuan Ren, Jihong Wu, Haiyang Lu. Effects of electroacupuncture preconditioning on behavioural and peripheral blood EPCs in cerebral ischaemia-reperfusion injury rats[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2024, 10(02): 71-77.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhsjcswkdzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-9141.2024.02.002

https://zhsjcswkdzzz.cma-cmc.com.cn/EN/Y2024/V10/I02/71

Figures/Tables 7

References 18

[1]	《中国脑卒中防治报告2021》编写组.《中国脑卒中防治报告2021》概要[J].中国脑血管病杂志, 2023, 20(11): 783-792. DOI: 10.3969/j.issn.1672-5921.2023.11.009.
[2]	杨瑞瑞,谢鹏,程焱. VEGF动员内源性EPCs促进MCAO/R小鼠神经功能恢复[J].第三军医大学学报, 2010, 32(7): 647-650. DOI: 10.16016/j.1000-5404.2010.07.023.
[3]	王琪,王满侠,郑婷,等.内皮祖细胞在缺血性脑血管病中的研究进展[J].卒中与神经疾病, 2016, 23(4): 298-301. DOI: 10.3969/j.issn.1007-0478.2016.04.024.
[4]	Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke, 1989, 20(1): 84-91. DOI: 10.1161/01.str.20.1.84.
[5]	Che QQ, Huang T, Zhang YD, et al. Effect of miR-124 on neuronal apoptosis in rats with cerebral infarction through Wnt/β-catenin signaling pathway[J]. Eur Rev Med Pharmacol Sci, 2019, 23(15): 6657-6664. DOI: 10.26355/eurrev_201908_18556.
[6]	王梦洁,李炜健,宋心怡,等.改良标记点线栓法建立大鼠脑缺血再灌注模型[J].实验技术与管理, 2022, 39(10): 42-48. DOI: 10.16791/j.cnki.sjg.2022.10.008.
[7]	陈潞婷,彭姣姣,黄晓琳,等.电针通过调控NGF/TrkA信号通路改善脑缺血大鼠学习记忆障碍的研究[J].中国康复医学杂志, 2020, 35(2): 129-134. DOI: 10.3969/j.issn.1001-1242.2020.02.001.
[8]	龙漫,郑洲,陈泽斌.电针预处理防治大鼠脑缺血再灌注损伤的研究进展[J].中国临床研究, 2020, 33(1): 124-126. DOI: 10.13429/j.cnki.cjcr.2020.01.031.
[9]	邵田娱,丁敏芮,周秀,等.基于数据挖掘探究针刺督脉治疗急性缺血性中风的腧穴应用规律[J].中华中医药杂志, 2021, 36(2): 1118-1121.
[10]	刘建勋,林咸明.不同时程电针预处理对脑缺血大鼠血脑屏障功能的保护效应[J].上海针灸杂志, 2014, 33(12): 1169-1172. DOI: 10.13460/j.issn.1005-0957.2014.12.1169.
[11]	吴超,陈国仙,赵刘阳,等. PeriCam PSI血流灌注成像系统在脑缺血再灌注动物模型中的应用及评价[J].浙江大学学报(医学版), 2018, 47(1): 51-56. DOI: 10.3785/j.issn.1008-9292.2018.02.07.
[12]	关明函,薛志强.右美托咪定改善大鼠脑缺血再灌注后脑损伤的研究[J].中华神经创伤外科电子杂志, 2023, 9(5): 270-276. DOI: 10.3877/cma.j.issn.2095-9141.2023.05.003.
[13]	田园,杨海昌,李秀华,等.羟考酮预处理对老龄脑缺血再灌注损伤大鼠的脑保护的作用[J].中国临床药理学杂志, 2023, 39(17): 2517-2521. DOI: 10.13699/j.cnki.1001-6821.2023.17.018.
[14]	印婷,陈海萍,汪文,等.电针预处理对急性脑梗死大鼠神经功能损伤及大脑皮层Cx 43蛋白表达的影响[J].针刺研究, 2019, 44(7): 497-500. DOI: 10.13702/j.1000-0607.190067.
[15]	金成日,姜奥,张小卿.电针对脑缺血再灌注大鼠行为学影响实验研究[J].辽宁中医药大学学报, 2017, 19(8): 108-110. DOI: 10.13194/j.issn.1673-842x.2017.08.030.
[16]	项正兵,屈新辉,王万松,等.急性脑梗死患者内皮祖细胞动态变化及其对神经功能的影响[J].中国老年学杂志, 2015, 35(16): 4520-4524. DOI: 10.3969/j.issn.1005-9202.2015.16.044.
[17]	Ghani U, Shuaib A, Salam A, et al. Endothelial progenitor cells during cerebrovascular disease[J]. Stroke, 2005, 36(1): 151-153. DOI: 10.1161/01.Str.0000149944.15406.16.
[18]	张智申,董贝贝,谢克亮,等.血管内皮祖细胞-血管再生在缺血再灌注损伤中的作用探讨[J].天津医药, 2016, 44(6): 694-699, 650. DOI: 10.11958/20160042.

组别	只数	线栓即刻	缺血后2 h	再灌注10 min	再灌注20 min	再灌注30 min	F值	P值
假手术组	6	97.11±6.56	91.67±12.12	93.87±11.47	95.94±11.60	95.43±9.56	1.976	0.162
模型组	6	44.62±5.44^a	45.75±3.33^a	59.92±4.80^a	60.33±4.83^a	57.00±3.87^a	50.724	<0.001
EA组	6	52.13±3.88^ab	47.41±3.33^a	66.48±2.54^a	65.74±1.75^a	67.83±4.02^ab	106.045	<0.001
F值		137.810	60.161	30.194	34.293	48.095
P值		<0.001	<0.001	<0.001	<0.001	<0.001

组别	只数	线栓即刻	缺血后2 h	再灌注10 min	再灌注20 min	再灌注30 min	F值	P值
假手术组	6	97.11±6.56	91.67±12.12	93.87±11.47	95.94±11.60	95.43±9.56	1.976	0.162
模型组	6	44.62±5.44^a	45.75±3.33^a	59.92±4.80^a	60.33±4.83^a	57.00±3.87^a	50.724	<0.001
EA组	6	52.13±3.88^ab	47.41±3.33^a	66.48±2.54^a	65.74±1.75^a	67.83±4.02^ab	106.045	<0.001
F值		137.810	60.161	30.194	34.293	48.095
P值		<0.001	<0.001	<0.001	<0.001	<0.001

组别	只数	梗死体积
假手术组	9	0.00±0.00
模型组	9	29.01±3.52^a
EA组	9	23.67±3.53^ab
F值		232.017
P值		0.001

组别	只数	梗死体积
假手术组	9	0.00±0.00
模型组	9	29.01±3.52^a
EA组	9	23.67±3.53^ab
F值		232.017
P值		0.001

组别	只数	造模后1 d	造模后3 d	造模后7 d	造模后21 d	造模后28 d	F值	P值
假手术组	6	0	0	0	0	0	-	-
模型组	6	12.00±1.41^a	9.00±0.58^a	7.00±1.15^a	3.83±1.46^a	2.33±1.60^a	54.772	<0.001
EA组	6	9.33±0.94^ab	7.50±0.76^ab	4.83±1.57^ab	2.33±1.25^ab	1.33±0.94	50.638	<0.001
F值		206.154	380.455	50.620	15.150	5.968
P值		<0.001	<0.001	<0.001	<0.001	0.012

Please choose a citation manager

Content to export