Laminin targeted NT3 promotes traumatic brain injury repair

doi:10.3877/cma.j.issn.2095-9141.2016.01.007

Abstract

Abstract:

Objective

To construct and prepare laminin binding domain NT3(LBD-NT3), exploring its mechanism to promote brain injury repaire with increasing laminin.

Methods

Fourty-six rats were grouped into naive(8)、sham(8)、PBS(10)、NT3(10)、LBD-NT3(10) randomly. Following hydraulic strike, PBS or NT3 or LBD-NT3 was injected into the lesion. Behavioral test went on by the Morris water maze task. One month after the operation, the rats were perfused and executed, neuron and glial cell were compared by NeuN and GFAP separately with Immunofluorescence staining. Statistical analysis was made by SPSS 19.0.

Results

In the Laminin-NT3 group, rats performed better in Morris water maze task, P<0.05(compared with PBS), P>0.05(compared with na?ve、sham、NT3), but significantly better than NT3 group on 17^th、18^th day (P<0.05) . LBD-NT3 had more positive NeuN cells and and less GFAP expression than PBS、NT3 (P<0.05) . There was statistical significance among the groups.

Conclusion

LBD-NT3 could bind to the increasing laminin. Therefore, the brain lesion was smaller, neurons increased and glial cells decreased.

Key words: Laminin, Traumatic brain injury, Neurotrophin-3

Juan Fan, Zhenhai Zhang, Guoliang Zhang, Chunsen Shen, Yongchun Luo, Xiaomin Yuan, Pengfei Zhang, Yuanyuan Dang. Laminin targeted NT3 promotes traumatic brain injury repair[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2016, 02(01): 26-30.

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Figures/Tables 8

References 15

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?	分组
?	假手术组	空白对照组	PBS	NT3	LBD‐NT3
时间	64.862±3.682	64.331±5.689	84.593±3.005	73.309±3.362	63.987±3.196

?	分组
?	假手术组	空白对照组	PBS	NT3	LBD‐NT3
时间	64.862±3.682	64.331±5.689	84.593±3.005	73.309±3.362	63.987±3.196

?	分组
?	假手术组	空白对照组	PBS	NT3	LBD‐NT3
路程	579.82±67.28	576.34±108.77	830.37±42.77	771.74±42.54	544.21±64.08

?	分组
?	假手术组	空白对照组	PBS	NT3	LBD‐NT3
路程	579.82±67.28	576.34±108.77	830.37±42.77	771.74±42.54	544.21±64.08

?	分组
?	假手术组	空白对照组	PBS	NT3	LBD‐NT3
NeuN阳性细胞数	430.86±197.72	507±141.85	243.43±87.06	310.86±127.66	425.57±107.84

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