Effects of hyperbaric oxygen on the level of excitatory amino acids in the brain of rats with brain injury

doi:10.3877/cma.j.issn.2095-9141.2016.05.007

Abstract

Abstract:

Objective

To study the effect of hyperbaric oxygen on the level of excitatory amino acids in the brain of rats with brain injury.

Methods

By making the models of traumatic brain injury in rats and design randomized experimental group: model control group, high pressure group, high concentration oxygen group and hyperbaric oxygen group were treated with brain injury rats. The contents of glutamic acid (Glu) and aspartate (Asp) were measured in rats at third days, sixth days and ninth days respectively. The contents of glutamic acid (Glu) and aspartate (Asp) were measured in rats at third days, sixth days and ninth days respectively.

Results

The content of Glu and ASP in the brain tissue of rats with brain injury increased significantly and hyperbaric oxygen can be significantly reduced content of Glu and ASP. Compared with model control group, there were significant differences (P<0.05). The high pressure group and high concentration oxygen group could not significantly inhibit the expression of Glu and Asp. Compared with the model group had no significant difference (P>0.05).

Conclusion

Hyperbaric oxygen can significantly inhibit the release of excitatory amino acids in brain tissue of rats with traumatic brain injury and reduce the secondary brain damage after traumatic brain injury, while high pressure, high concentration of oxygen can not significantly inhibit the release of EAA.

Key words: Cerebral injury, Excitatory amino acids, Hyperbaric oxygen therapy

Shiqing He, Yunlong Zou, Beiping Lei. Effects of hyperbaric oxygen on the level of excitatory amino acids in the brain of rats with brain injury[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2016, 02(05): 289-292.

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References 15

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组别	例数	平均体重（g）
空白对照组	18	273±4.09
模型对照组	18	278±5.16
高气压组	18	272±5.24
高浓度氧组	18	266±4.63
高压氧组	18	270±3.98
F值	?	2.709
P值	?	>0.05

组别	例数	平均体重（g）
空白对照组	18	273±4.09
模型对照组	18	278±5.16
高气压组	18	272±5.24
高浓度氧组	18	266±4.63
高压氧组	18	270±3.98
F值	?	2.709
P值	?	>0.05

实验分组	标本例数	处理时间（d）	兴奋性氨基酸水平
实验分组	标本例数	处理时间（d）	谷氨酸	天冬氨酸
空白对照组	6	3	2.65±0.29^a	1.05±1.02^b
模型对照组	6	3	13.72±2.27^c	5.15±1.54^d
高气压组	6	3	12.34±1.95^e	5.01±1.77^f
高浓度氧组	6	3	12.56±2.51^g	4.98±1.82^h
高压氧组	6	3	4.43±1.53ⁱ	2.03±1.05^j
F值	?	?	45.48	10.44
P值	?	?	<0.01	<0.01

实验分组	标本例数	处理时间（d）	兴奋性氨基酸水平
实验分组	标本例数	处理时间（d）	谷氨酸	天冬氨酸
空白对照组	6	3	2.65±0.29^a	1.05±1.02^b
模型对照组	6	3	13.72±2.27^c	5.15±1.54^d
高气压组	6	3	12.34±1.95^e	5.01±1.77^f
高浓度氧组	6	3	12.56±2.51^g	4.98±1.82^h
高压氧组	6	3	4.43±1.53ⁱ	2.03±1.05^j
F值	?	?	45.48	10.44
P值	?	?	<0.01	<0.01

实验分组	标本例数	处理时间（d）	兴奋性氨基酸水平
实验分组	标本例数	处理时间（d）	谷氨酸	天冬氨酸
空白对照组	6	6	2.95±0.47^k	1.54±1.38^l
模型对照组	6	6	14.28±2.67^m	5.63±1.07ⁿ
高气压组	6	6	13.94±2.56^o	5.81±1.49^p
高浓度氧组	6	6	13.55±1.63^q	5.43±1.99^r
高压氧组	6	6	4.72±1.21^s	2.74±1.67^t
F值	?	?	51.58	9.58
P值	?	?	<0.01	<0.01

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