Effects of peripheral nerve injury on the proliferation and osteogenesis differentiation of bone marrow mesenchymal stem cells

doi:10.3877/cma.j.issn.2095-9141.2023.01.002

Abstract

Abstract:

Objective

To explore the effect of peripheral nerve injury on the proliferation and osteogenesis differentiation of bone mesenchymal stem cells (BMSCs) by establishing sciatic nerve injury model.

Methods

Twenty four-week-old, healthy, SPF grade male SD rats were selected to establish the sciatic nerve injury model of one side of the lower limb. Four weeks after operation, BMSCs were isolated from the healthy side (control group) and the operative side (experimental group), and cultured and proliferated in vitro. The surface markers of BMSCs were identified by flow cytometry, and the activity of cells in the two groups was detected by MTT assay; at the same time, P3 cells were taken for osteogenic differentiation, and the osteogenic differentiation ability of the two groups was compared by alizarin red staining and alkaline phosphatase (ALP) activity detection.

Results

The flow cytometry indicated that CD90 was positive and CD11b was negative in the two groups. MTT test showed that the growth rate of experimental group was lower than the control group. After BMSCs induced differentiation into osteoblasts, the absorbance value of calcified nodules and ALP activity in the experimental group were lower than those in the control group, and the difference was statistically significant (P<0.05).

Conclusion

Peripheral nerve injury are able to inhibit BMSCs proliferation and differentiation to osteoblasts.

Key words: Peripheral nerve injury, Bone mesenchymal stem cells, Osteoporosis differentiation

Ranran Liu, Qianqian Fang, Zewen Tang. Effects of peripheral nerve injury on the proliferation and osteogenesis differentiation of bone marrow mesenchymal stem cells[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2023, 09(01): 7-11.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhsjcswkdzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-9141.2023.01.002

https://zhsjcswkdzzz.cma-cmc.com.cn/EN/Y2023/V09/I01/7

Figures/Tables 5

References 21

[1]	何鹏,陈玉,高峰,等.失神经及制动后兔肱骨骨密度与生物力学性能的对比研究[J].中国骨质疏松杂志, 2019, 25(12): 1680-1684, 1720. DOI: 10.3969/j.issn.1006-7108.2019.12.003.
[2]	訾英,刘欣伟,李哲,等.应用鼠神经生长因子修复周围神经损伤对骨质疏松的影响[J].中国组织工程研究, 2014, 18(51): 8233-8237. DOI: 10.3969/j.issn.2095-4344.2014.51.007.
[3]	Wang ML, Rivlin M, Graham JG, et al. Peripheral nerve injury, scarring, and recovery[J]. Connect Tissue Res, 2019, 60(1): 3-9. DOI: 10.1080/03008207.2018.1489381.
[4]	冼群泽,季兴华,霍建忠,等.骨髓间充质干细胞对骨质疏松的治疗作用[J].中国骨质疏松杂志, 2021, 27(11): 1694-1698. DOI: 10.3969/j.issn.1006-7108.2021.11.027.
[5]	Gao Q, Wang L, Wang S, et al. Bone marrow mesenchymal stromal cells: identification, classification, and differentiation[J]. Front Cell Dev Biol, 2022, 9: 787118. DOI: 10.3389/fcell.2021.787118.
[6]	袁伟,赵金柱.失用性骨质疏松症的发病机制及预防治疗进展[J].中国组织工程研究, 2013, 17(15): 2795-2802. DOI: 10.3969/j.issn.2095-4344.2013.15.020.
[7]	Zhou X, Cao H, Guo J, et al. Effects of BMSC-derived EVs on bone metabolism[J]. Pharmaceutics, 2022, 14(5): 1012. DOI: 10.3390/pharmaceutics14051012.
[8]	王琰,王艳. Wnt信号通路在周围神经损伤后骨质疏松中的研究进展[J].中国康复理论与实践, 2020, 26(8): 930-935. DOI: 10.3969/j.issn.1006-9771.2020.08.011.
[9]	Kerschan-Schindl K. Prevention and rehabilitation of osteoporosis[J]. Wien Med Wochenschr, 2016, 166(1-2): 22-27. DOI: 10.1007/s10354-015-0417-y.
[10]	Muñoz M, Robinson K, Shibli-Rahhal A. Bone health and osteoporosis prevention and treatment[J]. Clin Obstet Gynecol, 2020, 63(4): 770-787. DOI: 10.1097/GRF.0000000000000572.
[11]	黄昌钊,王锐英,胡译文.失神经化骨质疏松动物模型的建立与评价[J].重庆医学, 2018, 47(26): 3368-3371. DOI: 10.3969/j.issn.1671-8348.2018.26.002.
[12]	唐志宏,段浩,钟宗雨,等.间充质干细胞移植治疗骨质疏松症的机制[J].中国组织工程研究, 2022, 26(19): 3090-3094. DOI: 10.12307/2022.391.
[13]	李振均,刘文超,段传志,等. BMSCs移植对脑缺血恢复期大鼠神经功能缺损的改善作用及Nogo-A表达的影响[J].中华神经医学杂志, 2018, 17(2): 117-123. DOI: 10.3760/cma.j.issn.1671-8925.2018.02.003.
[14]	马明,刘小龙,贾更新,等.间充质干细胞治疗骨质疏松的研究进展和未来展望[J].中国骨质疏松杂志, 2022, 28(6): 904-911. DOI: 10.3969/j.issn.1006-7108.2022.06.022.
[15]	Zhang L, Peng LP, Wu N, et al. Development of bone marrow mesenchymal stem cell culture in vitro[J]. Chin Med J (Engl), 2012, 125(9): 1650-1655.
[16]	孔亚敏,严隽陶,李华伟.动物实验周围神经损伤模型的研究进展[J].中国现代医学杂志, 2021, 31(10): 48-53. DOI: 10.3969/j.issn.1005-8982.2021.10.010.
[17]	Yang Y, Zhou J, Liang C, et al. Effects of highly selective sensory/motor nerve injury on bone metabolism and bone remodeling in rats[J]. J Musculoskelet Neuronal Interact, 2022, 22(4): 524-535.
[18]	李强强,谢亚东,杨国清,等.骨髓间充质干细胞成骨分化的研究进展[J].医学综述, 2022, 28(3): 434-438. DOI: 10.3969/j.issn.1006-2084.2022.03.004.
[19]	王枫,赵立奇,姬宇辉,等.神经生长因子与被动运动对失神经大鼠骨代谢指标的影响[J].中国医药科学, 2022, 12(1): 35-38. DOI: 10.3969/j.issn.2095-0616.2022.01.010.
[20]	Aspray TJ, Hill TR. Osteoporosis and the ageing skeleton[J]. Subcell Biochem, 2019, 91: 453-476. DOI: 10.1007/978-981-13-3681-2_16.
[21]	徐如祥.神经生长因子的神经损伤修复机制及作用[J].中华神经创伤外科电子杂志, 2016, 2(1): 1-4. DOI: 10.3877/cma.j.issn.2095-9141.2016.01.001.

组别	第1天	第2天	第3天	第4天	第5天	第6天	第7天
实验组	0.39±0.03	0.41±0.02	0.48±0.04	0.52±0.03	0.55±0.03	0.59±0.03	0.61±0.02
对照组	0.50±0.01	0.55±0.03	0.60±0.03	0.65±0.02	0.68±0.05	0.70±0.03	0.75±0.01
t值	6.025	-2.127	-4.170	-6.268	-3.867	-4.489	-2.911
P值	0.009	0.008	0.031	0.011	0.039	0.028	0.002

组别	第1天	第2天	第3天	第4天	第5天	第6天	第7天
实验组	0.39±0.03	0.41±0.02	0.48±0.04	0.52±0.03	0.55±0.03	0.59±0.03	0.61±0.02
对照组	0.50±0.01	0.55±0.03	0.60±0.03	0.65±0.02	0.68±0.05	0.70±0.03	0.75±0.01
t值	6.025	-2.127	-4.170	-6.268	-3.867	-4.489	-2.911
P值	0.009	0.008	0.031	0.011	0.039	0.028	0.002

组别	钙化结节	ALP
实验组	0.48±0.02	0.55±0.01
对照组	0.60±0.03	0.66±0.03
t值	-5.769	-6.024
P值	0.014	0.009

组别	钙化结节	ALP
实验组	0.48±0.02	0.55±0.01
对照组	0.60±0.03	0.66±0.03
t值	-5.769	-6.024
P值	0.014	0.009

Please choose a citation manager

Content to export