Protective function of Mcc950 on dendritic spine in mice after traumatic brain injury

doi:10.3877/cma.j.issn.2095-9141.2018.02.008

Abstract

Abstract:

Objective

To explore the function of Nod-like receptor protein 3 (NLRP3) inhibitor Mcc950 on the dendritic spine.

Methods

Establishing traumatic brain injury mouse model via pressure hitting in 60 C56BL/6J adult mice, then average the mice into three different groups based on manage process after brain injury: group control, group with normal saline, group Mcc950. After 8 months, detect the dendritic spine by Golgi-Cox staining and detect the dendritic spine regulatory protein by Western Blot.

Results

The number of 30 μm dendritic spine in group Mcc950 (32.08±1.831) is higher than group control (20.82±2.252) and group normal (19.38±1.623) saline significantly (group control vs group Mcc950: t=3.921, P=0.0007; group normal saline vs group Mcc950: t=5.187, P<0.0001). The expression level of dendritic regulatory protein PSD95, Profilin1 and GTPase HRAS are higher in group Mcc950 than group control and group normal saline, the difference is significantly (The times of expression level group Mcc950 compare to group control and group normal saline of these protein are: PSD95: 4.64, 4.34; Profilin1: 4.43, 4.47; GTPase HRAS: 2.97, 3.03. PSD95: group control vs group Mcc950: t=8.880, P=0.012, group normal saline vs group Mcc950: t=7.248, P=0.002; Profilin1: group control vs group Mcc950: t=6.654, P=0.022; group normal saline vs group Mcc950: t=5.340, P=0.033; GTPase HRAS: group control vs group Mcc950: t=5.092, P=0.036, group normal saline vs group Mcc950: t=4.469, P=0.047).

Conclusion

Mcc950, a NLRP3 inhibitor, plays a protective role in dendritic spine number after traumatic brain injury in mice.

Key words: Mcc950, Inflammasome, Nod-like receptor protein 3, Dendritic spine

Yi Wu, Yong Liu, Xu Chen, Chenghui Liu. Protective function of Mcc950 on dendritic spine in mice after traumatic brain injury[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2018, 04(02): 98-102.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhsjcswkdzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-9141.2018.02.008

https://zhsjcswkdzzz.cma-cmc.com.cn/EN/Y2018/V04/I02/98

Figures/Tables 2

References 14

[1]	Ismael S,Nasoohi S,Ishrat T. MCC950, the selective NLRP3 inflammasome inhibitor protects mice against traumatic brain injury[J]. J Neurotrauma, 2018, Epub ahead of print.
[2]	van der Heijden T,Kritikou E,Venema W, et al. NLRP3 inflammasome inhibition by Mcc950 reduces atherosclerotic lesion development in apolipoprotein E-deficient mice-brief report[J]. Arterioscler Thromb Vasc Biol, 2017, 37(8): 1457-1461.
[3]	van Hout GP,Bosch L,Ellenbroek GH, et al. The selective NLRP3-inflammasome inhibitor MCC950 reduces infarct size and preserves cardiac function in a pig model of myocardial infarction[J]. Eur Heart J, 2017, 38(11): 828-836.
[4]	Chin LH,Hsu YJ,Hsu SC, et al. The regulation of NLRP3 inflammasome expression during the development of cardiac contractile dysfunction in chronic kidney disease[J]. Oncotarget, 2017, 8(69): 113303-113317.
[5]	Wan Z,Wen W,Ren K, et al. Involvement of NLRP3 inflammasome in the impacts of sodium and potassium on insulin resistance in normotensive Asians[J]. Br J Nutr, 2018, 119(2): 228-237.
[6]	Torres MD,Garcia O,Tang C, et al. Dendritic spine pathology and thrombospondin-1 deficits in Down syndrome[J]. Free Radic Biol Med, 2018, 114: 10-14.
[7]	Padamsey Z,McGuinness L,Emptage NJ. Inhibition of lysosomal Ca(2+) signalling disrupts dendritic spine structure and impairs wound healing in neurons[J]. Commun Integr Biol, 2017, 10(5-6): e1344802.
[8]	郭国庆,查彩慧.树突棘AMPA受体转运的细胞骨架机制[J].暨南大学学报(自然科学与医学版), 2017, 38(1): 13-19.
[9]	Rosoklija GB,Petrushevski VM,Stankov A, et al. Reliable and durable Golgi staining of brain tissue from human autopsies and experimental animals[J]. J Neurosci Methods, 2014, 230: 20-29.
[10]	马丽娜,王蓉.大脑发育调节脑蛋白对神经元树突棘及突触可塑性的影响[J].中华老年心脑血管病杂志, 2013, 15(10): 1109-1111.
[11]	Alkam D,Feldman EZ,Singh A, et al. Profilin1 biology and its mutation, actin(g) in disease[J]. Cell Mol Life Sci, 2017, 74(6): 967-981.
[12]	Merchant B,Edelstein-Keshet L,Feng JJ. A Rho-GTPase based model explains spontaneous collective migration of neural crest cell clusters[J]. Dev Biol, 2018, Epub ahead of print.
[13]	Groswasser Z,Peled I. Survival and mortality following TBI[J]. Brain Inj, 2018, 32(2): 149-157.
[14]	梁芯,唐静,张杨,等.抑郁症CUS模型大鼠海马CA1区及CA3区内树突棘数目减少[J].中国组织化学与细胞化学杂志, 2016, 25(1): 59-66.

[1]	Yao Ma, Mingyi Yang, Ke Xu, Bo Hao, Peng Xu. Research advances in correlation between pyroptosis and rheumatoid arthritis [J]. Chinese Journal of Joint Surgery(Electronic Edition), 2022, 16(05): 586-591.
[2]	Jinyi Wang, Tingyi Bao. Predictive values of serum uric acid and NLRP3 inflammasome in pregnant women with hypertensive disorder complicating pregnancy with acute kidney injury [J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2021, 17(06): 663-670.
[3]	Chao Sun, Huaxing Huang, Xian Deng. Correlation analysis of nucleotide-binding oligomerization domain-like receptor protein 3 inflammasome, inflammatory factors, cysteine aspartic acid protease-1 and prognosis of burn combined with inhalation injury [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2020, 15(06): 428-433.
[4]	Xiang Zhuo, Bin Jiang, Wenjie Wang. Effect of radical operation for pancreatic cancer combined with vascular resection and reconstruction on the incidence of complications and quality of life in patients with pancreatic cancer [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2020, 14(02): 166-169.
[5]	Enting Zhuo, Lianchen Wang, Xuelian Chen, Guohong Fu, Jianmin Pan. Clinical significance of early regulation of intestinal flora in patients undergoing radical resection of colon cancer [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2020, 14(02): 162-165.
[6]	Qingguo Du, Fei Xue, Yansong Pu, Zhijun Mao, Dong Liu, Sida Liu, Jianhua Wang, Yanbin Long. Changes of oxidative stress and inflammatory factors of patients with advanced gastric cancer undergoing D2 radical gastrectomy [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2019, 13(06): 589-592.
[7]	Yuan Ji, Bajin Wei. Review of research progress on the role of NLRP3 inflammasome in the pathogenesis of adverse reactions of organ transplantation [J]. Chinese Journal of Transplantation(Electronic Edition), 2023, 17(05): 308-312.
[8]	Chaoxue Xiang, Fuxiang Li, Zhongli Zhu, Chang Liu, Jian Hu, Junya Li, Guoyun Zhu, Yuxi Song. Expression and role of NOD-like receptor thermal-protein domain-related protein 3 inflammasome in skeletal muscles of rats with ICU-acquired weakness [J]. Chinese Journal of Lung Diseases(Electronic Edition), 2020, 13(02): 198-203.
[9]	Lianlian Lei, Li Li, Jing Bi. The protective effect and mechanism of ghrelin on acute lung injury in mice [J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2021, 11(02): 83-89.
[10]	Kun Chi, Zhangning Fu, Chengcheng Song, Xiaodong Geng, Chao Liu, Guangyan Cai, Xiangmei Chen, Quan Hong. High uric acid caused pyroptosis of endothelial cells through the TXNIP/NLRP3 pathway [J]. Chinese Journal of Kidney Disease Investigation(Electronic Edition), 2021, 10(02): 81-89.
[11]	Jingyi Sun, Xuyan Liu, Wei Jiang. Research progress on the mechanism of podocyte injury mediated by NLRP3 inflammasome [J]. Chinese Journal of Kidney Disease Investigation(Electronic Edition), 2020, 09(06): 267-271.
[12]	Liya Ji, Ang Ji, Zhengli Di, Jing Xiong, Zhiqin Liu, Xiuyun Xue, Xiaowei Fei, Yanan Dou, Li Wang. Effect of folic acid on Parkinson's like injury of HT22 cells through the NLRP3/ASC/Caspase-1 signaling pathway [J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2022, 08(04): 196-203.
[13]	Shuo Jiang, Mengnan Wang, Huiying Zhao, Xiaoxia Guo, Huixia Wang, Youzhong An. cGAS/STING regulates inflammation of human pulmonary microvascular endothelial cells via NLRP3 inflammasome [J]. Chinese Journal of Critical Care & Intensive Care Medicine(Electronic Edition), 2021, 07(03): 233-240.
[14]	Wenjun Wu, Sha Sha, Cong Tian, Jian Zhu. Association between NLRP3 gene polymorphisms and inflammatory marks in elderly patients with acute myocardial infarction [J]. Chinese Journal of Clinicians(Electronic Edition), 2022, 16(03): 241-245.
[15]	Jinhong Zhou, Shumei Zeng, Lingqin Zhao, Xianying Cheng, Wenjun Li. Expression and significance of NOD-like receptor family protein 3 in middle-aged and elderly patients with endometrioid adenocarcinoma [J]. Chinese Journal of Geriatrics Research(Electronic Edition), 2020, 07(04): 16-20.

Please choose a citation manager

Content to export