Study on the effect of quercetin on inflammation and apoptosis in experimental intracerebral hemorrhage model in rats

doi:10.3877/cma.j.issn.2095-9141.2017.02.008

Abstract

Abstract:

Objective

To investigate the effect of quercetin on neuronal functional recovery in intracerebral hemorrhage (ICH) rats and its underlying mechanisms.

Methods

In the present study, we established an ICH model by injecting type VII bacterial collagenase (0.5 U) into the central striatum of male Sprague-Dawley rats. The animals were randomized to four groups: sham-operation group; ICH+vehicle group; ICH+5 mg/kg quercetin group; and ICH+50 mg/kg quercetin group. The expression levels of inflammatory cytokines in the brain tissue were assayed by real-time PCR, and cell apoptosis was assayed by TUNEL staining 3 d after model establishment.

Results

It was found that the expression levels of inflammation markers and the number of apoptotic cells were reduced significantly in ICH rats receiving quercetin in 50 mg/kg quercetin group.

Conclusion

These results confirmed the therapeutic efficacy of quercetin in repairing brain injury, probably by inhibiting inflammatory response and apoptosis, thus promoting nerve functional restoration.

Key words: Intracerebral hemorrhage, Quercetin, Inflammation, Apoptosis

Lanjuan Xu, Bo Yi, Baolin Li. Study on the effect of quercetin on inflammation and apoptosis in experimental intracerebral hemorrhage model in rats[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2017, 03(02): 93-97.

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Figures/Tables 7

References 13

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?	基因引物序列扩增产物大小
IL?1β	上游：5 ′ ?ATGGCAACTGTCCCTGAACT?3 ′	330 bp
IL?1β	下游：5 ′ ?GTCATCATCCCACGAGTCAC?3 ′	330 bp
IL?6	上游：5 ′ ?CTTGGGACTGATGTTGTTGAC?3 ′	390 bp
IL?6	下游：5 ′ ?CTCTGAATGACTCTGGCTTTG?3 ′	390 bp
TNF?α	上游：5 ′ ?TGGTGGTTTGCTACGACG?3 ′	230 bp
TNF?α	下游：5 ′ ?CTCCAGAACTCCAGGCGG?3 ′	230 bp

?	基因引物序列扩增产物大小
IL?1β	上游：5 ′ ?ATGGCAACTGTCCCTGAACT?3 ′	330 bp
IL?1β	下游：5 ′ ?GTCATCATCCCACGAGTCAC?3 ′	330 bp
IL?6	上游：5 ′ ?CTTGGGACTGATGTTGTTGAC?3 ′	390 bp
IL?6	下游：5 ′ ?CTCTGAATGACTCTGGCTTTG?3 ′	390 bp
TNF?α	上游：5 ′ ?TGGTGGTTTGCTACGACG?3 ′	230 bp
TNF?α	下游：5 ′ ?CTCCAGAACTCCAGGCGG?3 ′	230 bp

组别	例数	1h	72 h	t值	P值
假手术组	24	0	0	?	?
脑出血组	24	9.57±2.43	8.07±2.57	2.078	0.0434
脑出血+槲皮素5 mg/kg组	24	9.45±2.58	7.71±2.02	2.601	0.0124
脑出血+槲皮素50 mg/kg组	24	9.51±2.43	2.57±1.86^ab	11.110	0.0001

组别	例数	1h	72 h	t值	P值
假手术组	24	0	0	?	?
脑出血组	24	9.57±2.43	8.07±2.57	2.078	0.0434
脑出血+槲皮素5 mg/kg组	24	9.45±2.58	7.71±2.02	2.601	0.0124
脑出血+槲皮素50 mg/kg组	24	9.51±2.43	2.57±1.86^ab	11.110	0.0001

组别	例数	脑组织水含量（%）	TUNEL阳性细胞数（个）
假手术组	6	70.03±0.98	29.3±6.8
脑出血组	6	80.96±1.45	124.7±21.1
脑出血+槲皮素5 mg/kg组	6	80.42±1.29	119.7±22.7
脑出血+槲皮素50 mg/kg组	6	76.12±1.04^ab	79.6±19.9^ab

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