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中华神经创伤外科电子杂志 ›› 2018, Vol. 04 ›› Issue (06) : 353 -356. doi: 10.3877/cma.j.issn.2095-9141.2018.06.009

所属专题: 文献

基础研究

Toll样受体4基因缺失导致小鼠运动平衡障碍
朱健伟1, 李益飞2, 王兆涛3, 贾伟强4, 陈晨5, 汪继5, 顾应江1, 徐如祥5,()   
  1. 1. 646000 四川泸州,西南医科大学附属中医医院神经外科
    2. 518036 深圳,北京大学深圳医院神经外科
    3. 510220 广州,广州医科大学附属第二医院神经外科
    4. 550000 贵阳,贵州省人民医院神经外科
    5. 100700 北京,陆军总医院附属八一脑科医院
  • 收稿日期:2018-09-26 出版日期:2018-12-15
  • 通信作者: 徐如祥
  • 基金资助:
    西南医科大学青年科学基金(2017-ZRQN-188)

Loss of TLR4 impairs motor balance in mice

Jianwei Zhu1, Yifei Li2, Zhaotao Wang3, Weiqiang Jia4, Chen Chen5, Ji Wang5, Yingjiang Gu1, Ruxiang Xu5,()   

  1. 1. Department of Neurosurgery, Affiliated TCM Hospital of Southwest Medical University, Luzhou 646000, China
    2. Department of Neurosurgery, Affiliated Shenzhen Hospital of Perking University, Shenzhen 518036, China
    3. Department of Neurosurgery, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510220, China
    4. Department of Neurosurgery, Guizhou Provincial People’s Hospital, Guiyang 550000, China
    5. Affiliated Bayi Brain Hospital, PLA Army General Hospital, Beijing 100700, China
  • Received:2018-09-26 Published:2018-12-15
  • Corresponding author: Ruxiang Xu
  • About author:
    Corresponding author: Xu Ruxiang, Email:
引用本文:

朱健伟, 李益飞, 王兆涛, 贾伟强, 陈晨, 汪继, 顾应江, 徐如祥. Toll样受体4基因缺失导致小鼠运动平衡障碍[J]. 中华神经创伤外科电子杂志, 2018, 04(06): 353-356.

Jianwei Zhu, Yifei Li, Zhaotao Wang, Weiqiang Jia, Chen Chen, Ji Wang, Yingjiang Gu, Ruxiang Xu. Loss of TLR4 impairs motor balance in mice[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2018, 04(06): 353-356.

目的

分析Toll样受体4(TLR4)基因缺失对小鼠运动平衡能力的影响。

方法

通过运动平衡能力测试相关的行为学测试,检测并比较TLR4基因缺失(TLR4-/-)小鼠和其同窝对照的野生型(TLR4+/+)小鼠在运动平衡能力测试相关的行为学中的表现差异,并进一步分析与运动平衡能力密切相关的小脑结构的变化。

结果

TLR4-/-小鼠在足迹实验中的表现与野生型小鼠比较,差异无统计学意义(P>0.05),但在加速转棒实验中的表现则明显优于野生型小鼠(P<0.05),而在梯踏步实验中的表现则较野生型小鼠更差(P<0.05)。H&E染色结果显示,TLR4-/-小鼠的小脑结构并未发生明显改变,但小脑分子层的厚度较野生型小鼠却有所减少(P<0.05)。

结论

TLR4基因缺失可导致小鼠运动平衡能力受损。

Objective

To analysis the effection of Toll-like receptor 4 (TLR4) on motor balance in mice.

Methods

Compared the difference of performance in motor balance related behaviors tests between TLR4-/- mice and its littermates (TLR4+/+), and analysis the effection of TLR4 loss on cerebellar structures.

Results

TLR4-/- mice exhibited no difference in the footprint test (P>0.05), but a better performance in accelerating-rotarod test compared with TLR4+/+ mice (P<0.05). In addition, TLR4-/- mice show poor performances in Ladder rung task than their wildtype counterparts (P<0.05). The results from H&E staining revealed that cerebellar structure has no changes in TLR4-/- mice, but the thickness of molecular layers is decreased (P<0.05).

Conclusion

Loss of TLR4 can impair motor coordination in mice.

表1 2组小鼠的运动平衡能力实验结果(
图1 TLR4-/-小鼠与野生型对照小鼠小脑切片H&E染色结果
表2 2组小鼠的小脑形态学分析结果(
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