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中华神经创伤外科电子杂志 ›› 2017, Vol. 03 ›› Issue (06) : 360 -364. doi: 10.3877/cma.j.issn.2095-9141.2017.06.009

所属专题: 文献

基础研究

青蒿琥酯调节Claudin-5基因表达影响胶质瘤细胞力学特性的研究
连世忠1, 冯靖1, 苗旺1, 郭庚1, 王宏勤1, 胡晓玲2, 宋彬3, 徐勇明1, 徐茗4, 黄训5, 师如意2,()   
  1. 1. 030001 太原,山西医科大学第一医院神经外科
    2. 030001 太原,山西医科大学基础医学院
    3. 030001 太原,山西医科大学第一医院肿瘤科
    4. 030001 太原,山西医科大学第一临床医学院
    5. 510630 广州,暨南大学附属第一医院骨科
  • 收稿日期:2017-02-27 出版日期:2017-12-15
  • 通信作者: 师如意
  • 基金资助:
    山西省基础研究计划(2015011095)

Effects of Artesunate on gene Claudin-5 expression which could influent cell mechanical properties in Glioma

Shizhong Lian1, Jing Feng1, Wang Miao1, Geng Guo1, Hongqin Wang1, Xiaoling Hu2, Bin Song3, Yongming Xu1, Ming Xu4, Xun Huang5, Ruyi Shi2,()   

  1. 1. Department of Neurosurgery, First Hospital of Shanxi medical University, Taiyuan 030001, China
    2. Basic Medical School, Shanxi Medical University, Taiyuan 030001, China
    3. Department of Oncology, First Hospital of Shanxi Medical University, Taiyuan 030001, China
    4. First Clinical Medical College, Shanxi Medical University, Taiyuan 030001, China
    5. Department of Orthopedics, the First Affiliated Hospital of Ji’nan University, Guangzhou 510630, China
  • Received:2017-02-27 Published:2017-12-15
  • Corresponding author: Ruyi Shi
  • About author:
    Corresponding author: Shi Ruyi, Email:
引用本文:

连世忠, 冯靖, 苗旺, 郭庚, 王宏勤, 胡晓玲, 宋彬, 徐勇明, 徐茗, 黄训, 师如意. 青蒿琥酯调节Claudin-5基因表达影响胶质瘤细胞力学特性的研究[J]. 中华神经创伤外科电子杂志, 2017, 03(06): 360-364.

Shizhong Lian, Jing Feng, Wang Miao, Geng Guo, Hongqin Wang, Xiaoling Hu, Bin Song, Yongming Xu, Ming Xu, Xun Huang, Ruyi Shi. Effects of Artesunate on gene Claudin-5 expression which could influent cell mechanical properties in Glioma[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2017, 03(06): 360-364.

目的

检测青蒿琥酯(Art)对胶质瘤SHG44细胞中Claudin-5基因表达及细胞力学特性的影响。

方法

利用多聚酶链式反应法、原子力显微镜检测法、trans-well法分别检测Art对Claudin-5基因表达、SHG44细胞力学特性及细胞迁移能力等方面的影响。

结果

Art可显著上调细胞间紧密连接蛋白Claudin-5基因表达;同时可以明显改变SHG44细胞力学特性:30 mg/l Art处理后的细胞粘附力显著升高(2600±400)pN vs(3800±300)pN,细胞弹力显著下降(26±6)MPa vs(4.7±1.6) MPa,细胞表面粗糙度大幅上升(0.150±0.013)μm vs(0.330±0.022)μm,可有效抑制SHG44细胞的迁移。

结论

实验结果表明Art可以通过影响Claudin-5基因表达,从而改变SHG44细胞力学特性进而发挥其抗癌作用,是潜在的胶质瘤治疗新药。

Objective

To observe the effects of Artesunate (Art) on expression of gene Claudin-5 in Glioma and cell mechaniacal properties by different biological detecting methods.

Methods

After treated by Art, the expression of gene Claudin-5 in SHG44 had been detected by polymerase chain reaction method; cell migration had been detected by trans-well method. And we used atomic force microscope to observe the change of cell mechanical properties.

Results

The Art could upregulate the expression of gene Claudin-5, which is the intercellular tight junction protein, leading to change of the mechanical properties of SHG44 cells to inhibit SHG44 cell migration effectively. The cell adhesive force increased significantly after 30 mg/l Art treatment (2600 ± 400) pN vs (3800 ± 300) pN. And the cell elasticity decreased significantly after 30 mg/l Art treatment (26 ± 6) MPa vs (4.7 ± 1.6) MPa. The membrane surface roughness of SHG44 increased significantly (0.15 ± 0.013) μm vs (0.33 ± 0.022) μm after 30 mg/l Art treatment.

Conclusion

The Art, which could be a potential anti-tumor drug, could affect the biological characteristics of SHG44 through regulating the expression of gene Claudin-5 to resist Glioma.

表1 GaPDHClaudin-5基因引物序列表
图1 青蒿琥酯处理前后SHG44细胞中Claudin-5表达情况
图2 原子力显微镜检测青蒿琥酯处理前后SHG44细胞力学特性及细胞微观结构变化情况
图3 原子力显微镜检测青蒿琥酯处理前后SHG44细胞力学特性变化情况
图4 青蒿琥酯处理前后SHG44细胞迁移情况
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