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中华神经创伤外科电子杂志 ›› 2017, Vol. 03 ›› Issue (03) : 155 -158. doi: 10.3877/cma.j.issn.2095-9141.2017.03.007

所属专题: 文献

基础研究

MicroRNA-195对胶质瘤U251细胞株CCND1表达的影响
徐勇明1, 任书娴1, 常璐1, 曲志钊2, 李亚娜2, 王宏勤2,()   
  1. 1. 030001 太原,山西医科大学研究生院
    2. 030001 太原,山西医科大学第一医院神经外科
  • 收稿日期:2017-03-09 出版日期:2017-06-15
  • 通信作者: 王宏勤
  • 基金资助:
    国家自然科学基金(81470115)

Effect of microRNA-195 on the expression of CCND1 in glioma U251 cell

Yongming Xu1, Shuxian Ren1, Lu Chang1, Zhizhao Qu2, Yana Li2, Hongqin Wang2,()   

  1. 1. Department of Neurosurgery, The Graduate School of Shanxi Medical University, Taiyuan 030001, China
    2. Department of Neurosurgery, The First Hospital of Shanxi Medical University, Taiyuan 030001, China
  • Received:2017-03-09 Published:2017-06-15
  • Corresponding author: Hongqin Wang
  • About author:
    Corresponding author: Wang Hongqin, Email:
引用本文:

徐勇明, 任书娴, 常璐, 曲志钊, 李亚娜, 王宏勤. MicroRNA-195对胶质瘤U251细胞株CCND1表达的影响[J]. 中华神经创伤外科电子杂志, 2017, 03(03): 155-158.

Yongming Xu, Shuxian Ren, Lu Chang, Zhizhao Qu, Yana Li, Hongqin Wang. Effect of microRNA-195 on the expression of CCND1 in glioma U251 cell[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2017, 03(03): 155-158.

目的

研究microRNA-195对胶质瘤U251细胞株CCND1表达的影响。

方法

通过脂质体Lipofectamin 2000瞬时转染法将microRNA-195过表达、microRNA-195抑制表达转染胶质瘤细胞株U251。以空白转染组作为空白对照组(K组)、microRNA-195过表达转染组为M组、microRNA-195抑制表达转染组为I组。Real-time PCR检测转染前后microRNA-195的含量以确保转染效率;再通过real-time PCR检测转染前后CCND1mRNA的表达变化;western blotting检测胶质瘤细胞U251中CCND1的表达水平。

结果

Real-time PCR结果显示M组CCND1mRNA的表达低于I组(M:758.264<I:1398.04),western blotting实验显示M组相对I组CCND1表达减少(M:2029<I:22365.66)。

结论

MicroRNA-195对胶质瘤U251细胞株CCND1表达有影响。

Objective

To research the effect of microRNA-195 on the expression of CCND1 in glioma U251 cell.

Methods

MicroRNA-195 mimics, microRNA-195 inhibitors were transfected into human glioma U251 cell by lipofectamine 2000 transient transfection. The microRNA-195 mimics transfection group was M group, and the microRNA-195 inhibitors transfection group was group I(blank control group, K group). Real-time PCR was used to detect the content of microRNA-195 before and after transfection to ensure the transfection efficiency. The expression of CCND1 mRNA was detected by real-time PCR. The expression of CCND1 in glioma U251 cells was detected by western blotting.

Results

Real-time PCR results showed: the expression of CCND1 mRNA in group M was lower than that in group I (M:758.264<I:1398.04) ; western blotting: the expression of CCND1 was decreased in M group compared with I group(M:2029<I:22365.66).

Conclusion

MicroRNA-195 has an effect on the expression of glioma U251 cell line CCND1.

图1 各组microRNA-195相对表达量
图2 各组CCND1mRNA相对表达量
图3 以β-actin为参照western blotting检测各组CCND1蛋白的表达
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