Protective effect of focal cerebral hypothermia on traumatic brain injury model

doi:10.3877/cma.j.issn.2095-9141.2019.02.009

Abstract

Abstract:

Objective

To explore the protective effect of focal cerebral hypothermia on traumatic brain injury (TBI) model in SD rats and the underlying mechanisms.

Methods

Fifteen male SD rats were randomly divided into a sham group, a non-cooling group, and a cooling group, with 5 in each group. The TBI model was made by non-cooling group and cooling group. Three groups of experiments were performed simultaneously. After trauma, the mice were treated with hypothermia for 3 h and rewarmed for 3 h. Rat blood gas and cortical brain electricity were detected during the experiment. Rats were sacrificed at the end of rewarming, and brain tissue was stained with TTC or HE to evaluate brain death. The cerebral edema was evaluated and the expression of related proteins was detected by Western blot.

Results

Sham group and non-cooling group were increased in metabolism by externally stimulated brain tissue, and metabolism of cooling group is low (P<0.05). TTC and HE staining showed that the area of brain death and cell death in the cooling group were less than those in the non-cooling group (P<0.05). Focal hypothermia after TBI significantly reduces epileptic spikes in the cerebral cortex (P<0.05). This incomplete inhibition persists during rewarming, and hypothermia treatment reduces the expression of GABA_B1R (P<0.05). Brain edema in the cooling group was significantly improved compared with the non-cooling group (P<0.05) and expression of AQP4 protein in the cooling group was significantly lower (P<0.05).

Conclusion

Focal hypothermia has a protective effect on TBI rats, which significantly reduce brain edema caused by TBI and inhibit epileptic spikes in the cerebral cortex. The underlying mechanism may be related to GABA_B1R and AQP4, respectively. It provides a safer, simpler and more effective method for clinical treatment of TBI.

Key words: Traumatic brain injury, Focal cerebral hypothermia, Epileptic spike, Edema

Xiaowei Fei, Ruxiang Xu, Minghai Wei, Yeting He. Protective effect of focal cerebral hypothermia on traumatic brain injury model[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2019, 05(02): 101-105.

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References 16

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