EID3 regulate the trans-differentiation process from human umbilical mesenchymal stem cell to neural stem-like cell

doi:10.3877/cma.j.issn.2095-9141.2018.01.009

Abstract

Abstract:

Objective

To explore the expression of E1A-like inhibitor of differentiation 3 (EID3) during the trans-differentiation of umbilical cord mesenchymal stem cell (UMSC) to neural stem-like cells (uNSCLs) and the relationship between EID3 and DNA methyltransferase (DNMT3A).

Methods

(1) To explore the characteristics of UMSC and uNSCLs via immunology experiments; (2) To explore expression and correlativity of EID3 and DNMT3A in UMSC, uNSCLs and NSC via epigenetic gene screen and Western Blot; (3) To explore the co-adjustment relationship between EID3 and DNMT3A in trans-differentiation from UMSC to uNSCLs via co-immunoprecipitation assay.

Results

(1) NSCs related mRNA level are highly expressed in uNSCLs significantly, the mRNA level including Nestin, Psx6, Vimentin, GFAP, Musashi1 and NeuroD1 are 3 to 13.2 times comparing to UMSCs (t=9.925, 14.089, 4.303, 8.994, 10.122, 22.327, 1.876; P=0.018, 0.0005, 0.026, 0.0003, 0.017, 0.002, 0.258); (2) After trans-differentiation of UMSCs into uNSCLs, the expression of EID3 is down-regulated, DNMT3A is up-regulated, the DNMT3A mRNA level in uNSCLs and NSCs is highly elevated comparing to UMSCs(t_uNSCLs=7.453, P_uNSCLs=0.006; t_NSCs=12.924, P_NSCs=0.001), the protein level are the same, EID3 is declining in expression but DNMT3A is increased in expression after this trans-differentiation process; (3) EID3 and DNMT3A are co-localized in cell nucleus and EID3 interact with DNMT3A directly in uNSCLs, as well as the exogenous EID3 and DNMT3A via transfection.

Conclusion

EID3 is upregulated during trans-differentiation process from UMSCs to uNSCLs and accompany with DNMT3A down-regulated expression, EID3 may participate in this trans-differentiation process from UMSCs to uNSCLs.

Key words: Mesenchymal stem cell, Trans-differentiation, Methylation

Wenjin Chen, Liang Luo, Li Zhang, Qinwei Yin, Ruxiang Xu. EID3 regulate the trans-differentiation process from human umbilical mesenchymal stem cell to neural stem-like cell[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2018, 04(01): 33-39.

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References 22

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