Correlation study of the relationship between BDNF and its Val66Met gene polymorphism and cognition in patients with diffuse axonal injury

doi:10.3877/cma.j.issn.2095-9141.2022.01.003

Abstract

Abstract:

Objective

To investigate the characteristics of cognitive impairment in patients with three subtypes of serum brain-derived neurotrophic factor (BDNF) and its gene Val66Met polymorphism one month after diffuse axonal injury (DAI) (type Ⅱ) injury.

Methods

One hundred and six patients with DAI (type II) in the Neurosurgery Department of Jinjiang Hospital from August 2015 to August 2020 were selected as the case group, and 105 healthy people who came to our hospital for physical examination in the same period were selected as the control group. The second edition of Loewenstein occupational therapy cognitive assessment (LOTCA) and Montreal cognitive assessment (MoCA) were used to evaluate the cognitive function of the control group and the case group at 1 month after injury; The levels of serum BDNF in the two groups were measured by enzyme-linked immunosorbent assay; Polymerase chain reaction restriction fragment length polymorphism analysis BDNF Val66Met gene polymorphism; The correlation between the overall cognitive function and BDNF and BDNF Val66Met gene polymorphism was analyzed by multiple stepwise regression.

Results

The concentration of serum BDNF of the same gene subtype in the case group was lower than that in the control group 1 month after injury, and the difference was statistically significant (P<0.05). The serum BDNF concentration of Val/Val subtype was significantly higher than that of Val/Met and Met/Met subtype in case group (P<0.05), but there was no significant difference in serum BDNF concentration between Val/Met and Met/Met subtypes (P>0.05). The LOTCA and MoCA scores of the three gene subtypes in the case group were lower than those in the control group 1 month after injury (P<0.05). The score of Val/Val subtype in case group was significantly higher than that of Val/Met and Met/Met (P<005), but there was no significant difference in the scores of Val/Met and Met/Met subtypes (P>0.05). The overall cognitive level of DAI (typeⅡ) had a linear regression relationship with BDNF Val66Met gene polymorphism and BDNF concentration (F=11.417, P<0.001), and it had a certain relationship (|β|=0.966, 0.877; r=0.569, 0.579).

Conclusion

BDNF may affect cognitive function in patients with diffuse axonal injury. The BDNF Val66Met polymorphism maybe one of the genetic factors in the cognitive function after DAI.

Key words: Diffuse axonal injury (typeⅡ), Cognitive impairment, Brain derived neurotrophic factor, Val66Met gene polymorphism

Weichuan Dai, Xieli Guo, Wenhua Cai, Yanfei Zheng, Yuyan Zhu, Yingxian Chen. Correlation study of the relationship between BDNF and its Val66Met gene polymorphism and cognition in patients with diffuse axonal injury[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2022, 08(01): 11-17.

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References 24

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因素		病例组（n=106）	对照组（n=105）	χ²/t值	P值
性别				0.039	0.844
	男	64	62
	女	42	43
年龄（Mean±SD，岁）		36.1±11.8	36.4±10.7	0.023	0.982
教育程度				0.024	0.981
	小学	39	40
	中学	35	34
	高中（中专）	25	25
	大学及其以上	7	6
职业				0.057	0.956
	工人	35	36
	农民	29	29
	职员	20	18
	自由职业	10	11
	其他	12	11

因素		病例组（n=106）	对照组（n=105）	χ²/t值	P值
性别				0.039	0.844
	男	64	62
	女	42	43
年龄（Mean±SD，岁）		36.1±11.8	36.4±10.7	0.023	0.982
教育程度				0.024	0.981
	小学	39	40
	中学	35	34
	高中（中专）	25	25
	大学及其以上	7	6
职业				0.057	0.956
	工人	35	36
	农民	29	29
	职员	20	18
	自由职业	10	11
	其他	12	11

因素		病例组（n=97）	对照组（n=98）	χ²/t值	P值
性别				0.054	0.879
	男	58	57
	女	39	41
年龄（Mean±SD，岁）		35.2±12.8	36.9±11.7	0.968	0.334
教育程度				0.397	0.718
	小学	36	38
	中学	33	32
	高中（中专）	23	23
	大学及其以上	5	5
职业				0.272	0.800
	工人	33	34
	农民	27	28
	职员	18	17
	自由职业	8	10
	其他	11	9

因素		病例组（n=97）	对照组（n=98）	χ²/t值	P值
性别				0.054	0.879
	男	58	57
	女	39	41
年龄（Mean±SD，岁）		35.2±12.8	36.9±11.7	0.968	0.334
教育程度				0.397	0.718
	小学	36	38
	中学	33	32
	高中（中专）	23	23
	大学及其以上	5	5
职业				0.272	0.800
	工人	33	34
	农民	27	28
	职员	18	17
	自由职业	8	10
	其他	11	9

组别	例数	基因型			等位基因
组别	例数	Val/Val	Val/Met	Met/Met	Val	Met
病例组	97	27（32.99）	39（35.05）	31（31.96）	93（48.94）	101（52.06）
对照组	98	28（29.57）	38（38.78）	32（31.65）	94（47.96）	102（52.04）
χ²值		0.042			0.411
P值		0.979			0.641

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