3D printing collagen/chitosan scaffold adsorbed NT-3 improved locomotor function after spinal cord injury

doi:10.3877/cma.j.issn.2095-9141.2021.06.003

Abstract

Abstract:

Objective

To explore the effect of transplantation of 3D printing collagen/chitosan scaffold adsorbed neurotrophin-3 (NT-3) on improving locomotor function after spinal cord injury (SCI).

Methods

The 3D printing collagen/chitosan scaffold adsorbed NT-3 was prepared. Eighty adult female SD rats (220-250 g) were randomly divided into four groups: sham operation group, model group, 3D printing scaffold group and 3D printing scaffold-factor group, with 20 rats in each group. Sham operation group: the spinal cord was exposed to the spinal cord and directly sutured the endorachis without injury; Model group: only undergoes T10 spinal cord transection; 3D printing scaffold group T10 spinal cord transection is implanted with 3D printing collagen/chitosan; 3D printing scaffold-factor group: T10 spinal cord transection was implanted with 3D printing collagen/chitosan scaffold adsorbed by NT-3. 1-8 weeks after SCI, the BBB score and the inclined-grid climbing test were used to evaluate the locomotor function of rats 8 weeks after SCI. Diffusion tensor fiber tract imaging was performed to observe the recovery of spinal cord defect at 8 weeks after SCI.

Results

(1) 1-8 weeks after SCI, compared to sham operation group, model group and 3D printing scaffold group, implantation of 3D printing collagen/chitosan scaffold adsorbed by NT-3 could significantly improve the spinal cord BBB score and slope angle of injured rats (P<0.05). (2) 8 weeks after SCI, compared with the 3D printing scaffold and model groups, the 3D printing scaffold-factor group showed improvements in amplitude and latency in the motor and somatosensory evoked potentials of the left and right hind limbs (P<0.05). (3) The image of diffusion tensor fiber bundle imaging at 8 weeks after SCI proved that the number of regenerated nerve fibers in the SCI area in 3D printing scaffold-factor group was significantly more than that of the 3D printing scaffold and model groups.

Conclusion

3D printing collagen/chitosan scaffold adsorbed NT-3 can improve locomotor function after SCI, and has the potential to become an innovative and safe method for the repair of SCI.

Key words: Spinal cord injury, 3D printing, Collagen, Chitosan, Neurotrophin-3, Scaffold, Locomotor function, Diffusion tensor fiber bundle imaging

Zhao Sui, Feng Peng, Kai Yu, Xiaohu Yan, Ying Li, Lin Zhong, Xiaoyin Liu. 3D printing collagen/chitosan scaffold adsorbed NT-3 improved locomotor function after spinal cord injury[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2021, 07(06): 331-338.

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References 39

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组别	只数	孔隙率（%）	吸水率（%）	压缩模量（MPa）	压缩应力（kPa）
3D打印胶原蛋白支架	20	49.03±5.56	1298.45±39.34	1.77±0.14	1187.47±7.25
3D打印胶原蛋白/壳聚糖支架	20	64.88±4.50	1085.75±49.61	3.93±0.41	184.95±5.66
t值		9.910	15.023	22.208	45.236
P值		0.007	0.004	0.002	0.003

组别	只数	孔隙率（%）	吸水率（%）	压缩模量（MPa）	压缩应力（kPa）
3D打印胶原蛋白支架	20	49.03±5.56	1298.45±39.34	1.77±0.14	1187.47±7.25
3D打印胶原蛋白/壳聚糖支架	20	64.88±4.50	1085.75±49.61	3.93±0.41	184.95±5.66
t值		9.910	15.023	22.208	45.236
P值		0.007	0.004	0.002	0.003

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