NHE1 inhibitor improves temozolomide-resistance of human glioblastoma cells

doi:10.3877/cma.j.issn.2095-9141.2021.05.002

Abstract

Abstract:

Objective

To analyze the effect of Na⁺/H⁺ exchanger 1 (NHE1) inhibitor Cariporide on improving temozolomide (TMZ)-resistance of human glioblastoma (GBM) cells.

Methods

Each group of cells was treated with TMZ resistant (TR) cell lines U87/TR and U251/TR, Cariporide which established in the early stage of this research group. The NHE1 activity was detected with acetoxymethyl ester of 2’,7’-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein fluorescent probe. The cell viability was analyzed by CCK-8 and the cell survival rate was calculated.

Results

The TMZ-resistances of U87/TR and U251/TR were confirmed by CCK-8 method. The NHE1 activity and cell survival rate of U87/TR and U251/TR were significantly higher than those of parental U87 and U251 cells, respectively (P<0.05). The NHE1 activities of Cariporide-treated U87/TR and U251/TR cells were still significantly higher than their parental cells, they were significantly lower than U87/TR and U251/TR cells without Cariporide, respectively (P<0.05). Under the conditions cultured at 325 μmol/L TMZ, the cell survival rate of Cariporide-treated U87/TR and U251/TR cells remained significantly higher than their parental cells, they were significantly lower than U87/TR and U251/TR cells without Cariporide, respectively (P<0.05).

Conclusion

Inhibition of NHE1 activity with Cariporide could partially improve the TMZ-resistance of GBM cells.

Key words: Glioblastoma, Temozolomide, Drug resistance, NHE1 inhibitor

Mingyang Sun, Yankun Liu, Si Chen, Yufeng Li, Yuhui Li. NHE1 inhibitor improves temozolomide-resistance of human glioblastoma cells[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2021, 07(05): 261-265.

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References 13

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