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Chinese Journal of Neurotraumatic Surgery(Electronic Edition) ›› 2021, Vol. 07 ›› Issue (03): 161-169. doi: 10.3877/cma.j.issn.2095-9141.2021.03.008

• Basic Research • Previous Articles     Next Articles

Transcriptome sequencing analysis: study on the PI3K, ERK and P38 signaling pathways and metabolic relationship in mice with Parkinson’s disease

Zihua Liu1, Degui Wang2, Xiaojuan Li3, Dongrong Tang3, Wen Wang3, Junyi Chen3, Jianlin Wang1,()   

  1. 1. Department of Zoology and Biomedical Science, School of Life Sciences, Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, Lanzhou University, Lanzhou 730000, China
    2. Department of Anatomy and Histology, Lanzhou University, School of Basic Medical Sciences, Lanzhou 730000, China
    3. Department of Blood Transfusion, Second Hospital of Lanzhou University, Lanzhou 730030, China
  • Received:2020-08-18 Online:2021-06-15 Published:2021-07-08
  • Contact: Jianlin Wang

Abstract:

Objective

To study the changes in metabolism and signaling pathway related factors in mice with Parkinson’s disease (PD).

Methods

Transcriptome sequencing and Q-PCR were performed in A53T mice compared with normal C57BL/6 (WT) mice. Quality control was done by Trimmomatic software. Genome alignment was done by hisat2 software. The expression of gene FPKM was done by cufflinks software. The difference expression was calculated by htseq-count software. Standardized data was done by R package software. Differential gene screening, cluster analysis and functional enrichment are analyzed by GO and KEGG enrichment. The results were verified by Q-PCR.

Results

The expression of Lipoid metabolism-related genes Botoine palmityltransferases 1A (CPT1), nucleic acid metabolism-related genes (Nme7), glucose metabolism-related gene hexosaccharide kinase 1 (hexokinase 1) and adenosine-triphosphate (ATP) decreased in A53T mice compared with WT mice; also the expression of caM, GluR and IP3R decreased in A53T mice compared with WT mice; the expression of D2R increased in A53T mice compared with WT mice; the expression of signaling pathway-related genes P38, ERK5 and IP3R decreased in A53T mice compared with WT mice.

Conclusion

Metabolism decreased in mice with PD through the PI3k, ERK and P38 signaling pathways.

Key words: Parkinson’s disease, Adenosine-triphosphate, Calmodulin, Extracellular signal-regulated kinase 5, Hexokinase 1

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