Blockade of NHE1 inhibits the proliferation and invasion of human glioblastoma

doi:10.3877/cma.j.issn.2095-9141.2020.01.010

Abstract

Abstract:

Objective

To analyze the effection of Na⁺/H⁺ exchanger 1(NHE1) on proliferation and invasion abilities of oncogene BRAF wild type(BRAF^WT) and BRAF^V600E mutation type glioblastoma (GBM) cells.

Methods

The U251 (BRAF^WT) and AM38 (BRAF^V600E) GBM cells were treated with NHE1 inhibitor Cariporide, respectively. The cells were treated with acetoxymethyl ester of 2’,7’-Bis (2-carboxyethyl)-5(6)-carboxyfluorescein fluorescent probe and the fluorescence intensities at 440 nm and 490 nm were detected by ultraviolet spectrophotometer. The fluorescence intensity ratio was calculated to reflect NHE1 activity. The proliferative activity was detected by MTT and the invasion ability was detected by matrigel-Transwell assay.

Results

The NHE1 activity, proliferation and invasion abilities of AM38 cells were all significantly higher than those of U251 cells (P=0.006, 0.010, 0.047, respectively). The NHE1 activity, proliferation and invasion abilities of both Cariporide-treated U251 and Cariporide-treated AM38 cells were all markedly decreased compared with those of DMSO-treated U251 and DMSO-treated AM38 cells, respectively (U251: P=0.012, 0.023, 0.044, respectively; AM38: P=0.006, 0.001, 0.038, respectively).

Conclusion

Cariporide could effectively inhibit the proliferation and invasion abilities of BRAF^WT and BRAF^V600E mutation type GBM cells.

Key words: Glioblastoma, Na⁺/H⁺ exchanger 1 inhibitor, BRAF^WT, BRAF^V600E mutation, Proliferation, Invasion

Yuhui Li, Xiqing Zhao, Si Chen, Yan Liu, Yankun Liu, Yufeng Li. Blockade of NHE1 inhibits the proliferation and invasion of human glioblastoma[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2020, 06(01): 39-43.

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