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Chinese Journal of Neurotraumatic Surgery(Electronic Edition) ›› 2016, Vol. 02 ›› Issue (06): 350-354. doi: 10.3877/cma.j.issn.2095-9141.2016.06.007

Special Issue:

• Basic Research • Previous Articles     Next Articles

Tumourigenicity and immunogenicity of induced neural stem cell grafts versus induced pluripotent stem cell grafts in syngeneic mouse brain

Mou Gao1, Ruxiang Xu1,(), Minhui Xu1, Hui Yao1, Qin Dong1, Hongtian Zhang1, Zhijun Yang1, Yang Yang1, Jianwei Zhu1   

  1. 1. Affiliated Bayi Brain Hospital, General Hospital of PLA Army, Beijing 100700, China
  • Received:2016-09-26 Online:2016-12-15 Published:2016-12-15
  • Contact: Ruxiang Xu
  • About author:
    Corresponding author: Xu Ruxiang, Email:

Abstract:

Objective

To provide scientific basis for evaluating the tumourigenicity and immunogenicity of induced neural stem cells (iNSCs) and induced pluripotent stem cells (iPSCs) in syngeneic mouse brain.

Methods

A total of 1×106 C57BL/6 (B6) embryonic stem cells (ESCs), iPSCs, neural stem cells (NSCs) or iNSCs were separately transplanted into the motor cortex of syngeneic adult mouse with normal immunologic function. At 14 and 28 d post-implantation, animals were sacrificed for morphological analysis.

Results

Both iPSCs and ESCs were able to form tumours in the mouse brain, leading to tissue destruction along with immune cell infiltration. In contrast, no evidence of tumour formation, brain injury or immune rejection was observed with iNSCs or NSCs.

Conclusion

INSC grafts, which lacked any resulting tumourigenicity or immunogenicity, are safer than iPSC grafts.

Key words: Tumourigenicity, Immunogenicity, Induced neural stem cell, Induced pluripotent stem cell, Transplantation

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