The activation of low density lipoprotein receptor related protein-1 dependent cell signaling pathway promotes axon growth and repair of nerve injury

doi:10.3877/cma.j.issn.2095-9141.2015.02.010

Abstract

Abstract:

Objective

To investigate the effects of the low density lipoprotein receptors-1(LRP1) dependent signal pathway in axonal growth and nerve regeneration after injury.

Methods

Adding RBD, LRP1 agonist, when culture the cortical neurons of fetal rats, observing whether it can promote the growth of synapses and what the signal mechanism it is compared with the control group.In addition,observing the effects of RBD on the repair of nerve injury through building the spinal cord injury model of rats up and intrathecal inject the RBD.

Results

The activation of neurotrophic factor receptor TrkC,Akt signal transduction pathway and extracellular regulated protein kinase(ERK)which were induced after add RBD in cortical neuron in vitro. The results of quantitative analysis of expression between the intervention group and the control group were significantly increased(P＜0.05), the growth of neurite were promoted. The recovery of motor function after spinal cord injury scores were significantly improved after the intrathecal injection of RBD in mice.

Conclusion

The activation of signal pathway dependented by LRP1 can obviously promote the growth of axon and repair functions of rats after the spinal injury.

Key words: Low density lipoprotein receptor- related protein- 1, Spinal cord injuries, Ethology, Wound healing

Chao Li, Yangjia Ou, Jun Zhu. The activation of low density lipoprotein receptor related protein-1 dependent cell signaling pathway promotes axon growth and repair of nerve injury[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2015, 01(02): 31-35.

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Figures/Tables 3

References 17

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