Abstract:
Objective
To study the change of biochemical index of malondialdehyde (MDA)and glutathione(GSH)of moderate traumatic brain injury(TBI)rats model,which are the end products of lipid oxidation and antioxidant, respectively. To explore the relationship between these two chemicals and secondary brain injury.
Methods
48 adult male Sprague-Dawley rats were randomly assigned to 2 groups : moderate-TBI (mTBI, n=24) group and sham-TBI (sham, n=24) group.Hydraulic moderate TBI index was induced to mTBI group. The rats were killed 6 h and 24 h after injured respectively, the levels of MDA and GSH were examined by ELISA enzymictechnology to analyze the different expression among 2 groups and to evaluate the oxidative stress injury of the rats after traumatic brain injury. The data was analyzed by SPSS 19.0 statistical analysis software, the concentration of GSH and MDA among 2 groups was tested by independent-samples t Test.
Results
Brain contusion and subarachnoid hemorrhage could be observed in mTBI rats, in brain sections with HE staining, nuclear pyknosis and vesicular neurons could be detected. However, no similar injury could be found in sham group.The concentration of MDA in mTBI group was significantly higher than that in sham group (in 6 h, level of mTBI 8.34±2.03μg/g, level of sham 3.92±1.20μg/g, t=6.493,P=0.000), and the concentration displayed a significant upregulated trend as time went by post TBI (in 24 h, level of mTBI 12.74±2.44μg/g, level of sham 3.96±1.18μg/g, t=11.222, P=0.000). The concentration of GSH in mTBI group was significantly lower than that in sham group (in 6 h, level of mTBI 2.65±0.63μg/g, level of sham 4.90±0.56μg/g, t=9.247, P=0.000), and the concentration displayed a significant downtrend as time went by post TBI (in 24 h, level of mTBI 2.20±0.62μg/g,level of sham 4.88±0.55μg/g, t=11.202, P=0.000).
Conclusions
The injury level of hydraulic TBI model can be evaluated,moderate closed brain trauma resulted in brain tissue pathological changes and oxidative stress injury.The index of oxidative stress was correlated with the injury period.Blockage of the process of the oxidative stress in the early stage post TBI may play an important role in neuroprotective effect.
Key words:
Traumatic brain injury,
Traumatology,
Sprague-Dawley rats,
Oxidative stress,
Malondialdehyde,
Glutathione
Hengzhou Lin, Meng Zhang, Baodong Chen, Xianjian Huang, Qiusheng Zhang, Tao Ji, Yi He, Weiping Li. Experimental study on oxidative stress in rats after moderate fluid-percussion brain injury[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2015, 01(01): 7-10.