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中华神经创伤外科电子杂志 ›› 2021, Vol. 07 ›› Issue (03) : 156 -160. doi: 10.3877/cma.j.issn.2095-9141.2021.03.007

颅脑与脊髓损伤

单细胞测序分析技术在小胶质细胞表型异质性研究中的最新进展
蔡霖1, 龚秋源1, 王伟1, 田恒力1,()   
  1. 1. 200233 上海,上海交通大学附属第六人民医院神经外科
  • 收稿日期:2021-03-04 出版日期:2021-06-15
  • 通信作者: 田恒力
  • 基金资助:
    国家自然科学基金(81974189,81671207); 上海市科委科技创新行动计划项目(19411968100)

Recent advances of single-cell sequencing technology in microglia phenotypic heterogeneity research

Lin Cai1, Qiuyuan Gong1, Wei Wang1, Hengli Tian1,()   

  1. 1. Department of Neurosurgery, Shanghai Jiaotong University Affiliated Sixth People’s Hospital, Shanghai 200233, China
  • Received:2021-03-04 Published:2021-06-15
  • Corresponding author: Hengli Tian
引用本文:

蔡霖, 龚秋源, 王伟, 田恒力. 单细胞测序分析技术在小胶质细胞表型异质性研究中的最新进展[J]. 中华神经创伤外科电子杂志, 2021, 07(03): 156-160.

Lin Cai, Qiuyuan Gong, Wei Wang, Hengli Tian. Recent advances of single-cell sequencing technology in microglia phenotypic heterogeneity research[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2021, 07(03): 156-160.

小胶质细胞(MG)是中枢神经系统(CNS)中的天然免疫巨噬细胞,在神经系统的发育和成熟过程中发挥着重要作用。MG具有显著的表型异质性,可以面对不同的环境变化因素产生一系列反应,进而维持CNS的稳定。目前,仍缺少一种有效且无偏倚的高通量检测技术来评估MG在时间及空间分布中的异质性。随着近年来单细胞技术的发展,以单细胞RNA测序和空间质谱分析为代表的一类高新技术手段极大地促进了对MG异质性的认识和理解。本文将对目前单细胞检测技术在MG异质性方面的研究成果作一综述。

Microglia (MG) are the innate macrophages in the central nervous system (CNS) that play a key role in the processes of development and adulthood of the nervous system. Although MG have been shown to manifest significant phenotypical heterogeneity, which allows them to make a wide range of responses to environmental change for the maintenance of CNS homeostasis. So far, an effective and unbiased and high-throughput method is still lacking to assess the spatial and temporal distribution heterogeneity of MG. The recent emergence of novel single-cell techniques, such as single-cell RNA sequencing and cytometry by time-of-flight mass spectrometry, have greatly promoted our understanding of MG heterogeneity. This article reviews the current knowledge about single-cell sequencing technology in MG heterogeneity research.

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