探讨JAZF1对脑胶质母细胞瘤（GBM）细胞侵袭、迁徙的作用及相关作用机制。

通过挖掘TCGA数据库中不同级别胶质瘤患者JAZF1的转录水平差异以及患者生存曲线差异，然后通过科内收集的胶质瘤样本提取总蛋白进行Western blot实验研究JAZF1蛋白水平在胶质瘤不同级别中的差异，通过Trans-well实验、细胞划痕实验研究JAZF1对胶质瘤细胞侵袭和迁移的影响，通过Western blot实验研究JAZF1对胶质瘤细胞侵袭和迁徙能力的关键分子的影响。

（1）Ⅳ级胶质瘤中JAZF1的mRNA、蛋白水平的表达上相较于Ⅱ级和Ⅲ级胶质瘤明显更高，差异具有统计学意义（P<0.05）；（2）JAZF1高表达组患者的中位生存期为13.0个月，而低表达组中位生存期为30.2个月，差异具有统计学意义（P<0.05）；（3）JAZF1表达下降后，U87M细胞的侵袭和迁徙能力均显著下降，差异具有统计学意义（P<0.05）；（4）干扰JAZF1的表达后，p-Akt、MMP9、MMP2的表达水平显著下调，差异均具有统计学意义（P<0.05），而Akt的表达变化不明显，差异无统计学意义（P>0.05）。

JAZF1在GBM中高表达且显著缩短了患者的中位生存期，通过调控Akt的磷酸化水平进一步调控相应信号通路下游的MMP9、MMP2的表达影响GBM的侵袭和迁徙能力。

To investigate the expression of JAZF1 in glioblastoma (GBM) and its effect of invasion and migration in GBM and the related mechanism.

By digging through TCGA database in different grade gliomas JAZF1 transcription level differences and differences in survival curve, and then by the glioma samples collected in the division of the total protein imprinting experiments JAZF1 protein levels in glioma differences of different levels, through the Trans-well experiment and scratched cell research JAZF1 influence on glioma cell invasion and migration, through experiments protein imprinting JAZF1 on glioma cell invasion and migration ability of the influence of key molecules.

(1) The mRNA and protein level of JAZF1 in grade Ⅳ glioma (GBM) are significantly higher than grade Ⅱ and grade Ⅲ glioma (P<0.05); (2) The median survival time in JAZF1 high expression group is 13.0 months, but 30.2 months in JAZF1 low expression group, this difference is significant (P<0.05); (3) When JAZF1 expression in U87M is silenced, the ability of invasion and migration is degressive significantly; (4) After JAZF1 expression was disturbed, p-Akt, MMP9 and MMP2 expression levels were significantly down-regulated, with statistically significant differences (P<0.05), while Akt expression was not significantly changed (P>0.05).

JAZF1 is high expression in GBM and shorten the median survival time significantly, and JAZF1 regulate the expression level of p-Akt and related downstream protein expression level, MMP9 and MMP2, to control the ability of invasion and migration in GBM.