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中华神经创伤外科电子杂志 ›› 2024, Vol. 10 ›› Issue (01) : 6 -15. doi: 10.3877/cma.j.issn.2095-9141.2024.01.002

基础研究

miR-145-5p抑制造釉细胞型颅咽管瘤细胞增殖、侵袭和SOX9/β-catenin表达
夏可顺1, 黄耀辉1, 王茂1, 刘志勇2, 谭博1, 宋鹏1, 印晓鸿1,†()   
  1. 1. 628099　四川广元，川北医学院附属广元市中心医院神经外科
    2. 610044　成都，四川大学华西医院神经外科
  • 收稿日期:2023-08-25 出版日期:2024-02-15
  • 通信作者: 印晓鸿

miR-145-5p inhibits proliferation, invasion and SOX9/β-catenin expression of adamantinomatous craniopharyngioma cells

Keshun Xia1, Yaohui Huang1, Mao Wang1, Zhiyong Liu2, Bo Tan1, Peng Song1, Xiaohong Yin1,†()   

  1. 1. Department of Neurosurgery, Guangyuan Central Hospital Affiliated to North Sichuan Medical College, Guangyuan 628099, China
    2. Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610044, China
  • Received:2023-08-25 Published:2024-02-15
  • Corresponding author: Xiaohong Yin
  • Supported by:
    Key Clinical Specialty Construction Project of Sichuan Province (2022)
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引用本文：

夏可顺, 黄耀辉, 王茂, 刘志勇, 谭博, 宋鹏, 印晓鸿. miR-145-5p抑制造釉细胞型颅咽管瘤细胞增殖、侵袭和SOX9/β-catenin表达[J]. 中华神经创伤外科电子杂志, 2024, 10(01): 6-15.

Keshun Xia, Yaohui Huang, Mao Wang, Zhiyong Liu, Bo Tan, Peng Song, Xiaohong Yin. miR-145-5p inhibits proliferation, invasion and SOX9/β-catenin expression of adamantinomatous craniopharyngioma cells[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2024, 10(01): 6-15.

目的

探究miR-145-5p对造釉细胞型颅咽管瘤（aCP）细胞增殖、侵袭和凋亡的影响及作用机制。

方法

选取2019年9月至2022年9月在四川大学华西医院和广元市中心医院神经外科术中切除的aCP和乳头状颅咽管瘤（pCP)各5例。采用实时荧光定量PCR（qRT-PCR）检测aCP和pCP中miR-145-5p、SOX9、CTNNB1（β-catenin基因）mRNA的表达差异，采用免疫荧光及Western blot检测SOX9、β-catenin蛋白的表达；通过CCK8实验、Transwell小室及流式细胞术分别检测颅咽管瘤细胞的增殖、侵袭和凋亡水平。将aCP细胞分为aCP组（空白对照，未转染）、aCP+miR-145-5p control组（阴性对照）、aCP+miR-145-5p mimic组（过表达），研究miR-145-5p对aCP细胞的增殖、侵袭和凋亡及SOX9、β-catenin表达的影响。

结果

与pCP相比，aCP细胞中miR-145-5p mRNA表达明显降低，SOX9、CTNNB1 mRNA、SOX9及胞核β-catenin蛋白表达明显增高，胞质β-catenin蛋白表达略降低，细胞增殖和侵袭显著增强，差异均有统计学意义（P<0.05）。aCP组、aCP+miR-145-5p control组和aCP+miR-145-5p mimic组在miR-145-5p、SOX9、CTNNB1 mRNA，SOX9、胞核β-catenin、胞质β-catenin蛋白表达以及细胞增殖和侵袭方面比较，差异均有统计学意义（P<0.05）。两两比较显示，aCP+miR-145-5p mimic组较其他2组miR-145-5p mRNA表达显著升高，细胞增殖和侵袭减弱，凋亡增加，SOX9、CTNNB1 mRNA表达明显降低；aCP+miR-145-5p mimic组较aCP+miR-145-5p control组SOX9、胞核β-catenin蛋白表达明显降低，胞质β-catenin蛋白表达略升高，差异均有统计学意义（P<0.05）。

结论

miR-145-5p可能通过下调SOX9、β-catenin抑制aCP的增殖、侵袭能力，促进细胞凋亡。

关键词: 造釉细胞型颅咽管瘤, 乳头状颅咽管瘤, miR-145-5p, SOX9, β-catenin, 增殖, 侵袭, 凋亡
Objective

To investigate the effects of miR-145-5p on the proliferation, invasion, and apoptosis of adamantinomatous craniopharyngioma (aCP) cells and its mechanism of action.

Methods

Five cases of aCP and five cases of papillary craniopharyngioma (pCP) resected at Neurosurgery Department of West China Hospital of Sichuan University and Guangyuan Central Hospital from September 2019 to September 2022 were selected. The expression differences of miR-145-5p, SOX9, and CTNNB1 (β-catenin gene) mRNA in aCP and pCP were detected by real-time fluorescence quantitative PCR (qRT-PCR). The expression of SOX9 and β-catenin proteins were detected by immunofluorescence and Western blot. The proliferation, invasion, and apoptosis levels of craniopharyngioma cells were measured through CCK8 assay, Transwell chamber, and flow cytometry, respectively. aCP cells were divided into aCP group (blank control, untransfected), aCP+miR-145-5p control group (negative control), and aCP+miR-145-5p mimic group (overexpression) to study the effects of miR-145-5p on the proliferation, invasion, and apoptosis of aCP cells and on the expression of SOX9 and β-catenin.

Results

Compared with pCP, aCP cells showed significantly reduced expression of miR-145-5p mRNA and increased expression of SOX9、CTNNB1 mRNA, SOX9、nuclear β-catenin proteins, and a decreased in cytoplasmic β-catenin protein expression, concurrently, cell proliferation and invasion were significantly enhanced, with all differences being statistically significant (P<0.05). There were differences among the aCP group, aCP+miR-145-5p control group, and aCP+miR-145-5p mimic group in terms of miR-145-5p, SOX9, and CTNNB1 mRNA expression, as well as SOX9, nuclear β-catenin, and cytoplasmic β-catenin protein expression, and in cell proliferation and invasion (P<0.05). Pairwise comparisons between groups showed that the aCP+miR-145-5p mimic group had significantly increased expression of miR-145-5p mRNA, weakened proliferation and invasion, and increased apoptosis compared to the other two groups. The expression of SOX9 and CTNNB1 mRNA was significantly reduced. Compared to the aCP+miR-145-5p control group, the expression of SOX9 and nuclear β-catenin proteins decreased, while cytoplasmic β-catenin protein expression increased in the aCP+miR-145-5p mimic group, with all differences being statistically significant (P<0.05).

Conclusion

miR-145-5p may inhibit the proliferation、invasion of aCP and promote cell apoptosis by down-regulating the SOX9 and β-catenin.

Key words: Adamantinomatous craniopharyngioma, Papillary craniopharyngioma, miR-145-5p, SOX9, β-catenin, Proliferation, Invasion, Apoptosis
表1 qRT-PCR所用引物序列
Tab.1 Primer sequences for qRT-PCR
名称 引物方向 序列（5’-3’）
miR-145-5p 正向 TGCGCGTCCAGTTTTCCCAGGAA
反向 CCAGTGCAGGGTCCGAGGTATT
U6 正向 CGCTTCGGCAGCACATATAC
反向 AAATATGGAACGCTTCACGA
SOX9 正向 ATGAAGATGACCGACGAGCA
反向 CAGTCGTAGCCTTTGAGCAC
CTNNB1 正向 CCAAGTGGGTGGTATAGAGG
反向 AGTCCATAGTGAAGGCGAAC
GAPDH 正向 TCAAGAAGGTGGTGAAGCAGG
反向 TCAAAGGTGGAGGAGTGGGT
图1 aCP和pCP原代细胞pan-CK表达水平（×400）aCP：造釉细胞型颅咽管瘤；pCP：乳头状颅咽管瘤
Fig.1 pan-CK expression in primary cells of aCP and pCP (×400)
图2 Transwell检测aCP和pCP原代细胞的侵袭能力（×200）A：pCP细胞；B：aCP细胞；aCP：造釉细胞型颅咽管瘤；pCP：乳头状颅咽管瘤
Fig.2 The invasion ability of aCP and pCP primary cells was detected by Transwell assay（×200）
图3 免疫荧光检测aCP和pCP细胞中SOX9、β-catenin表达（×200）A：aCP和pCP细胞中SOX9表达；B：aCP和pCP细胞中β-catenin表达；aCP：造釉细胞型颅咽管瘤；pCP：乳头状颅咽管瘤
Fig.3 Immunofluorescence detect the expression of SOX9 and β-catenin in aCP and pCP primary cells (×200)
图4 Western blot检测aCP和pCP原代细胞中SOX9、β-catenin表达水平A：SOX9、β-catenin表达的电泳条带图；B：SOX9、β-catenin表达水平比较；与pCP比较，aP<0.05；aCP：造釉细胞型颅咽管瘤；pCP：乳头状颅咽管瘤
Fig.4 Western blot detect the expression of SOX9 and β-catenin in aCP and pCP primary cells
表2 qRT-PCR检测miR-145-5p、SOX9及CTNNB1在aCP和pCP细胞中的表达（±s）
Tab.2 Expressions of miR-145-5p, SOX9 and CTNNB1 in aCP and pCP cells detected by qRT-PCR (Mean±SD)
名称 aCP pCP t值 P值
miR-145-5p 0.65±0.07 1.00±0.19 -3.865 0.012
SOX9 2.75±0.26 1.00±0.18 12.374 <0.001
CTNNB1 2.22±0.31 1.00±0.10 8.207 <0.001
图5 Transwell检测miR-145-5p对aCP细胞侵袭能力的影响（×400）A：aCP组；B：aCP+miR-145-5p control组；C：aCP+miR-145-5p mimic组；aCP：造釉细胞型颅咽管瘤
Fig.5 Transwell assay detect the effect of miR-145-5p on invasive ability of aCP cells（×400)
图6 流式细胞术检测miR-145-5p对aCP细胞凋亡的影响A：aCP组；B：aCP+miR-145-5p control组；C：aCP+miR-145-5p mimic组；aCP：造釉细胞型颅咽管瘤
Fig.6 Flow cytometry detect the effect of miR-145-5p on apoptosis of aCP cells
表3 miR-145-5p对aCP细胞增殖、侵袭和凋亡的影响（±s）
Tab.3 Effects of miR-145-5p on proliferation, invasion and apoptosis of aCP cells（Mean±SD）
组别 转染后miR-145-5p表达 CCK8增殖 Transwell侵袭 流式凋亡
aCP组 1.00±0.11 100.00±7.22 157.40±13.58 3.72±0.79
aCP+miR-145-5p control组 0.92±0.18 95.98±8.06 164.60±12.82 6.87±2.17
aCP+miR-145-5p mimic组 7.10±0.75ab 79.92±4.68ab 103.40±6.43ab 24.95±2.44 ab
F值 301.432 12.187 43.045 174.106
P值 <0.001 0.001 <0.001 <0.001
图7 免疫荧光检测miR-145-5p对aCP细胞中SOX9、β-catenin蛋白表达的影响（×400）A：3组SOX9免疫荧光图；B：3组β-catenin免疫荧光图；aCP：造釉细胞型颅咽管瘤
Fig.7 Immunofluorescence detect the effect of miR-145-5p on the expression of SOX9 and β-catenin in aCP cells (×400)
图8 Western blot检测miR-145-5p对aCP细胞中SOX9、β-catenin蛋白表达的影响A：3组细胞SOX9、β-catenin表达的电泳条带图；1：aCP组；2：aCP+miR-145-5p control组；3：aCP+miR-145-5p mimic组；B：3组细胞SOX9、β-catenin蛋白表达水平比较；与aCP组比较，aP<0.05；与aCP+miR-145-5p control组比较，bP<0.05；aCP：造釉细胞型颅咽管瘤
Fig.8 Western blot detect the effect of miR-145-5p on the expression of SOX9 and β-catenin protein in aCP cells
表4 miR-145-5p对aCP细胞中SOX9、CTNNB1 mRNA表达的影响
Tab.4 Effect of miR-145-5p on the expression of SOX9 and CTNNB1 mRNA in aCP cells
组别 SOX9 CTNNB1
aCP组 1.00±0.15 1.00±0.09
aCP+miR-145-5p control组 1.01±0.10 1.09±0.14
aCP+miR-145-5p mimic组 0.61±0.08ab 0.68±0.11ab
F值 12.191 17.444
P值 0.001 <0.001
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