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中华神经创伤外科电子杂志 ›› 2015, Vol. 01 ›› Issue (05) : 25 -29. doi: 10.3877/cma.j.issn.2095-9141.2015.05.007

所属专题: 文献

基础研究

骨髓间充质干细胞移植对血管性痴呆大鼠认知功能的影响
黑悦1, 樊才瑞2, 龙乾发3, 罗强3, 魏礼洲1, 伊西才1, 刘卫平1,()   
  1. 1. 710032 西安,第四军医大学西京医院神经外科
    2. 571100 海口,解放军第187医院神经外科
    3. 710003 西安,西安市中心医院神经外科
  • 收稿日期:2015-05-11 出版日期:2015-10-15
  • 通信作者: 刘卫平
  • 基金资助:
    陕西省科技统筹基金资助项目(2013KTCL03-08)

The effect of bone marrow mesenchymal stem cells transplantation on the cognitive ability in a rat model of vascular dementia

Yue Hei1, Cairui Fan2, Qianfa Long3, Qiang Luo3, Lizhou Wei1, Xicai Yi1, Weiping Liu1,()   

  1. 1. Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China
    2. Department of Neurosurgery, 187th Hospital of PLA, Haikou 571100, China
    3. Department of Neurosurgery, Xi’an central hospital, Xi’an Jiao Tong University, Xi’an 710003, China
  • Received:2015-05-11 Published:2015-10-15
  • Corresponding author: Weiping Liu
  • About author:
    Corresponding author: Liu Weiping, Email:
引用本文:

黑悦, 樊才瑞, 龙乾发, 罗强, 魏礼洲, 伊西才, 刘卫平. 骨髓间充质干细胞移植对血管性痴呆大鼠认知功能的影响[J]. 中华神经创伤外科电子杂志, 2015, 01(05): 25-29.

Yue Hei, Cairui Fan, Qianfa Long, Qiang Luo, Lizhou Wei, Xicai Yi, Weiping Liu. The effect of bone marrow mesenchymal stem cells transplantation on the cognitive ability in a rat model of vascular dementia[J]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2015, 01(05): 25-29.

目的

探究骨髓间充质干细胞(BMSCs)移植对血管性痴呆大鼠认知功能及海马CA1区域GAD67、GABABR1的表达的影响。

方法

成年雄性SD大鼠60只随机分为假手术组(n=20),模型组(n=20)和移植组(n=20)。结扎双侧颈总动脉建立大鼠血管性痴呆模型,2 h后BMSCs尾静脉移植,28 d后水迷宫检测认知变化,Western blotting和免疫组化分析大鼠海马CA1区GAD67和GABABR1的表达变化。

结果

(1)Western blotting和免疫组化结果显示,模型组相比假手术组在慢性期海马CA1区GAD67和GABABR1表达水平(蛋白水平以及免疫组化染色强度)下调,差异具有统计学意义(分别为1.347±0.357比2.237±0.956,t=5.478,P=0.0076;0.779±0.181比1.691±0.337,t=4.893,P=0.0073)。(2)移植组GAD67和GABABR1表达水平改变均比模型组轻微,差异具有统计学意义(分别为1.611±1.007比1.347±0.357,t=2.379,P=0.0373;1.009±0.257比0.779±0.181,t=2.678,P=0.0441)。(3)水迷宫认知功能测试显示,移植组比模型组潜伏期缩短(20.817±2.891比30.192±3.472,t=2.743,P=0.0442),且在游泳轨迹方面显示出更好的方向性。

结论

BMSCs移植可提升血管性痴呆大鼠认知功能并上调海马CA1区GAD67和GABABR1表达水平。

Objective

To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on bilateral common carotid artery occlusion (2VO)-induced cognitive impairments and expression of GAD67 and GABABR1 in the hippocampal CA1 subfield.

Methods

60 adult male SD rats were randomly divided into Sham group (n=20), group (n=20) and BMSCs group (n=20). BMSCs transplantation was performed 2 h after the surgery of 2VO. After 28 days, Morris Water Maze (MWM) test was performed to investigate the cognitive changes, and Western blot and immunohistochemistry were used to evaluate the expression of GAD67 and GABABR1 in the hippocampal CA1 subfield.

Results

(1)Western blot and immunohistochemistry showed that compared to sham group, the expression of GAD67 and GABABR1 (protein level and the intensity of immunohistochemical staining) lowered(1.347±0.357 vs. 2.237±0.956, t=5.478, P=0.0076; 0.779±0.181 vs. 1.691±0.337, t=4.893, P=0.0073, respectively). (2)while BMSCs transplants partly reversed these changes(1.611±1.007 vs. 1.347±0.357, t=2.379, P=0.0373; 1.009±0.257 vs. 0.779±0.181, t=2.678, P=0.0441, respectively). (3)MWM also showed that cognitive ability in the BMSCs group improved significantly (20.817±2.891 vs. 30.192±3.472, t=2.743, P=0.0442) compared to VaD group.

Conclusion

BMSCs transplantation could improve the expression of GAD67 and GABABR1 in the hippocampal CA1 subfield and restore the cognitive ability in a rat model of vascular dementia.

图1 大鼠水迷宫实验的逃避潜伏期
图2 大鼠水迷宫实验的游泳轨迹
图3 GAD67和GABABR1的免疫组化染色结果
图4 GAD67和GABABR1的Western blot结果
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