LAMP1对脑缺血再灌注大鼠小胶质细胞损伤和自噬的影响

doi:10.3877/cma.j.issn.2095-9141.2025.04.004

中华神经创伤外科电子杂志 ›› 2025, Vol. 11 ›› Issue (04) : 230 -237. doi: 10.3877/cma.j.issn.2095-9141.2025.04.004

基础研究

LAMP1对脑缺血再灌注大鼠小胶质细胞损伤和自噬的影响

艾孜买提江·吐尔逊¹, 玉苏甫·马合木提², 姜世豪³, 卡合尔曼·卡德尔¹, 买买提力·艾沙¹, 苏日青¹, 成晓江¹^,^†(

)

¹830000　乌鲁木齐，新疆医科大学第一附属医院神经外科
²310058　杭州，浙江大学脑科学与脑医学学院
³100053　北京，首都医科大学宣武医院神经外科

收稿日期:2024-10-29 出版日期:2025-08-15
通信作者: 成晓江

Effects of LAMP1 on microglial cell injury and autophagy in rats with cerebral ischemia-reperfusion injury

Tuerxun Aizimaitijiang¹, Mahemuti Yusufu², Shihao Jiang³, Kadeer Kaheerman¹, Aisha Maimaitili¹, Riqing Su¹, Xiaojiang Cheng¹^,^†()

¹Department of Neurosurgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, China
²School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou 310058, China
³Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China

Received:2024-10-29 Published:2025-08-15
Corresponding author: Xiaojiang Cheng
Supported by:
Xinjiang Key Laboratory of Neurological Disorder Research Fund(XJDX1711-2201)

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引用本文：

艾孜买提江·吐尔逊, 玉苏甫·马合木提, 姜世豪, 卡合尔曼·卡德尔, 买买提力·艾沙, 苏日青, 成晓江. LAMP1对脑缺血再灌注大鼠小胶质细胞损伤和自噬的影响[J/OL]. 中华神经创伤外科电子杂志, 2025, 11(04): 230-237.

Tuerxun Aizimaitijiang, Mahemuti Yusufu, Shihao Jiang, Kadeer Kaheerman, Aisha Maimaitili, Riqing Su, Xiaojiang Cheng. Effects of LAMP1 on microglial cell injury and autophagy in rats with cerebral ischemia-reperfusion injury[J/OL]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2025, 11(04): 230-237.

目的

探讨溶酶体相关膜蛋白1（LAMP1）对大鼠脑缺血再灌注（CIRI）后小胶质细胞（MG）损伤的作用，及对自噬的影响及机制。

方法

选取30只成年健康SD大鼠，采用短暂性大脑中动脉栓塞（transient middle cerebral artery occlusion, tMCAO）法建立大鼠模型，按照随机数字表法分为假手术组（Sham组）、手术组（tMCAO组）、干预组（tMCAO+CQ组），每组10只。3组大鼠术前均腹腔注射无菌生理盐水，tMCAO+CQ组于术前2 h注射氯喹（CQ）溶酶体抑制剂。每组取5只用于TTC染色，剩余5只取脑组织标本按序完成实验检测。取大鼠脑组织中缺血半暗带区组织，检测细胞中LAMP1蛋白的表达量。为探讨LAMP1在模型中致MG损伤和自噬作用，采用酶联免疫吸附测定（ELISA）试剂盒检测致炎症因子[白介素（IL）-6、肿瘤坏死因子-α（TNF-α）、IL-10]的表达，采用Western blot检测p62、Cathepsin B、Cathepsin D、LC3B蛋白的表达情况。此外，采用Longa分制法评估大鼠CIRI后24 h的神经功能，TTC染色测定脑梗死体积，HE染色观察脑组织病理学变化。

结果

相较于Sham组，tMCAO组和tMCAO+CQ组的Longa评分明显升高，脑梗死体积明显增加，且tMCAO+CQ组的Longa评分高于tMCAO组，脑梗死体积大于tMCAO组，差异均有统计学意义（P<0.05）。ELISA和Western blot检测结果显示，相较于Sham组，tMCAO组与tMCAO+CQ组的LAMP1蛋白表达明显下降，IL-6、TNF-α含量及LC3B、Baclin1蛋白表达水平明显升高，IL-10含量及LAMP1、p62、Cathepsin B及Cathepsin D蛋白表达水平明显降低，差异均有统计学意义（P<0.05）；相较于tMCAO组，tMCAO+CQ组LAMP1蛋白表达明显下降，IL-6、TNF-α含量及LC3B、Beclin1蛋白表达水平升高，p62和Cathepsin D蛋白表达水平降低，差异均有统计学意义（P<0.05），而Cathepsin B蛋白的表达水平比较，差异无统计学意义（P>0.05）。

结论

在大鼠CIRI模型中，抑制LAMP1对MG损伤有明显的加重作用，该机制可能与诱导自噬障碍有关。

关键词: 溶酶体相关膜蛋白1, 脑缺血再灌注损伤, 小胶质细胞, 自噬, 损伤

Objective

To observe the role of lysosomal-associated membrane protein 1 (LAMP1) in microglial (MG) injury in rats subjected to cerebral ischemic-reperfusion injury (CIRI) and to investigate the impact and mechanism of LAMP1 on autophagy in this model.

Methods

Thirty adult healthy SD rats were selected and the rat model was established using transient middle cerebral artery occlusion (tMCAO) method. The rats were randomly divided into sham group (Sham group), surgical group (tMCAO group), and intervention group (tMCAO+CQ group) according to the random number table method, with 10 rats in each group. Three groups of rats were intraperitoneally injected with sterile physiological saline before surgery, while the tMCAO+CQ group was injected with chloroquine (CQ) lysosome inhibitor 2 h before surgery. CIRI rat model was established, and Sham group was the surgical control group. Five samples were taken from each group for TTC staining, and the remaining five brain tissue specimens were collected for experimental testing in sequence. After collecting brain tissue and extracting the ischemic penumbra tissue, the expression of LAMP1 protein in cells was detected. To investigate the effects of LAMP1 on MG injury and autophagy in a model, enzyme-linked immunosorbent assay (ELISA) was used to detect inflammatory factors [interleukin (IL)-6, tumor necrosis factor alpha (TNF-α), IL-10], and Western blot was used to detect the expression of p62, Cathepsin B, Cathepsin D, and LC3B. In addition, the Longa scoring system was used to evaluate the neurological function of rats 24 h after CIRI, TTC staining was used to measure the volume of cerebral infarction, HE staining was used to observe pathological changes in brain tissue.

Results

Compared with the Sham group, the Longa scores of the tMCAO group and the tMCAO+CQ group were significantly increased, and the cerebral infarction volume was significantly increased. The Longa score of the tMCAO+CQ group was higher than that of the tMCAO group, and the cerebral infarction volume was larger than that of the tMCAO group, with statistical significance (P<0.05). The results of ELISA and Western blot analysis showed that compared with the Sham group, the expression of LAMP1 protein was significantly decreased in the tMCAO group and the tMCAO+CQ group, while the levels of IL-6, TNF-α, LC3B, and Baclin1 protein expression were significantly increased. The levels of IL-10 and p62, LAMP1, Cathepsin B, and Cathepsin D protein expression were significantly decreased, and the differences were statistically significant (P<0.05); Compared with the tMCAO group, the expression of LAMP1 protein in the tMCAO+CQ group decreased significantly, while the levels of IL-6, TNF-α, LC3B, and Beclin1 protein increased. The expression levels of p62 and Cathepsin D protein decreased, and the differences were statistically significant (P<0.05). However, there was no statistically significant difference in the expression levels of Cathepsin B protein (P>0.05).

Conclusions

Inhibiting LAMP1 exacerbates glial cell damage in the rat model and this mechanism may be related to the inhibition of autophagy.

Key words: Lysosomal-associated membrane protein 1, Cerebral ischemic-reperfusion injury, Microglia, Autophagy, Injury

表1 3组大鼠脑缺血再灌注损伤后Longa评分及脑梗死体积比较

Tab.1 Comparison of Longa score and cerebral infarct volume after cerebral ischemia-reperfusion injury in 3 groups of rats

组别	只数	Longa评分[分，M（P₂₅，P₇₅）]	脑梗死体积占比（%，±s）
Sham组	5	0	0
tMCAO组	5	2.00（2.00，2.50）^a	28.87±4.52^a
tMCAO+CQ组	5	3.00（2.00，3.00）^ab	40.25±4.77^ab
H/F值		8.333	149.474
P值		0.004	<0.001

表1 3组大鼠脑缺血再灌注损伤后Longa评分及脑梗死体积比较

Tab.1 Comparison of Longa score and cerebral infarct volume after cerebral ischemia-reperfusion injury in 3 groups of rats

组别	只数	Longa评分[分，M（P₂₅，P₇₅）]	脑梗死体积占比（%，±s）
Sham组	5	0	0
tMCAO组	5	2.00（2.00，2.50）^a	28.87±4.52^a
tMCAO+CQ组	5	3.00（2.00，3.00）^ab	40.25±4.77^ab
H/F值		8.333	149.474
P值		0.004	<0.001

图1 3组大鼠脑缺血再灌注损伤后脑组织TTC染色结果

Fig.1 TTC staining results of brain tissue after cerebral ischemia-reperfusion injury in 3 groups of rats

图2 3组大鼠脑组织坏死的HE染色结果（×200）A：Sham组；B：tMCAO组（箭头示神经元变性，胞核深染）；C：tMCAO+CQ组（红色箭头示组织水肿，间隙增大；黑色箭头示胞核固缩深染）；tMCAO：短暂性大脑中动脉栓塞；CQ：氯喹

Fig.2 HE staining results of brain tissue necrosis in 3 groups of rats (×200)

表2 3组大鼠脑组织中炎症因子含量比较（pg/mL）

Tab.2 Comparison of inflammatory factor content in brain tissue of 3 groups of rats (pg/mL)

组别	只数	IL-6（±s）	IL-10（±s）	TNF-α[M（P₂₅，P₇₅）]
Sham组	5	22.18±4.53	433.10±86.42	4.30（3.05，8.20）
tMCAO组	5	90.10±6.86^a	159.10±23.44^a	21.50（16.56，23.59）^a
tMCAO+CQ组	5	128.90±14.52^ab	77.82±7.23^ab	29.56（26.85，31.18）^ab
F/H值		80.427	157.340	12.500
P值		<0.001	<0.001	0.002

表2 3组大鼠脑组织中炎症因子含量比较（pg/mL）

Tab.2 Comparison of inflammatory factor content in brain tissue of 3 groups of rats (pg/mL)

组别	只数	IL-6（±s）	IL-10（±s）	TNF-α[M（P₂₅，P₇₅）]
Sham组	5	22.18±4.53	433.10±86.42	4.30（3.05，8.20）
tMCAO组	5	90.10±6.86^a	159.10±23.44^a	21.50（16.56，23.59）^a
tMCAO+CQ组	5	128.90±14.52^ab	77.82±7.23^ab	29.56（26.85，31.18）^ab
F/H值		80.427	157.340	12.500
P值		<0.001	<0.001	0.002

表3 3组大鼠脑组织中自噬溶酶体途径相关蛋白表达水平比较（

±s）

Tab.3 Comparison of protein expression levels related to autophagy lysosome pathway in brain tissues of 3 groups of rats (Mean±SD)

组别	只数	LAMP1	LC3B	p62	Beclin1	Cathepsin B	Cathepsin D
Sham组	5	2.765±0.074	0.649±0.024	1.574±0.091	1.072±0.084	2.060±0.141	1.972±0.068
tMCAO组	5	2.178±0.235^a	1.240±0.097^a	0.570±0.117^a	2.143±0.241^a	1.534±0.138^a	1.454±0.155^a
tMCAO+CQ组	5	0.862±0.034^ab	1.786±0.052^ab	0.261±0.036^ab	2.898±0.115^ab	1.513±0.094^a	1.277±0.117^ab
F值		230.679	382.255	123.738	160.126	29.907	46.149
P值		<0.001	<0.001	<0.001	<0.001	<0.001	<0.001

表3 3组大鼠脑组织中自噬溶酶体途径相关蛋白表达水平比较（

±s）

Tab.3 Comparison of protein expression levels related to autophagy lysosome pathway in brain tissues of 3 groups of rats (Mean±SD)

组别	只数	LAMP1	LC3B	p62	Beclin1	Cathepsin B	Cathepsin D
Sham组	5	2.765±0.074	0.649±0.024	1.574±0.091	1.072±0.084	2.060±0.141	1.972±0.068
tMCAO组	5	2.178±0.235^a	1.240±0.097^a	0.570±0.117^a	2.143±0.241^a	1.534±0.138^a	1.454±0.155^a
tMCAO+CQ组	5	0.862±0.034^ab	1.786±0.052^ab	0.261±0.036^ab	2.898±0.115^ab	1.513±0.094^a	1.277±0.117^ab
F值		230.679	382.255	123.738	160.126	29.907	46.149
P值		<0.001	<0.001	<0.001	<0.001	<0.001	<0.001

图3 3组大鼠脑组织中自噬溶酶体途径相关蛋白表达的Western blot电泳条带图

Fig.3 Western blot electrophoresis bands of autophagy lysosome pathway related protein expression in brain tissue of 3 groups of rats

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Effects of LAMP1 on microglial cell injury and autophagy in rats with cerebral ischemia-reperfusion injury

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Effects of LAMP1 on microglial cell injury and autophagy in rats with cerebral ischemia-reperfusion injury