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中华神经创伤外科电子杂志 ›› 2018, Vol. 04 ›› Issue (04) : 226 -230. doi: 10.3877/cma.j.issn.2095-9141.2018.04.009

所属专题: 文献

基础研究

Toll样受体4在小鼠小脑组织中的表达情况分析
朱健伟1, 李益飞2, 王兆涛2, 贾伟强2, 陈晨2, 顾应江1, 胡蓉1, 徐如祥2,()   
  1. 1. 646000 四川泸州,西南医科大学附属中医医院神经外科
    2. 100700 北京,陆军总医院附属八一脑科医院
  • 收稿日期:2018-04-02 出版日期:2018-08-15
  • 通信作者: 徐如祥

Expression of Toll-like receptor 4 in mouse cerebellum

Jianwei Zhu1, Yifei Li2, Zhaotao Wang2, Weiqiang Jia2, Chen Chen2, Yingjiang Gu1, Hu Rong1, Ruxiang Xu2,()   

  1. 1. Department of Neurosurgery, Affiliated TCM Hospital of Southwest Medical University, Luzhou 646000, China
    2. Affiliated Bayi Brain Hospital, The PLA Army General Hospital, Beijng 100700, China
  • Received:2018-04-02 Published:2018-08-15
  • Corresponding author: Ruxiang Xu
  • About author:
    Corresponding author: Xu Ruxiang, Email:
引用本文:

朱健伟, 李益飞, 王兆涛, 贾伟强, 陈晨, 顾应江, 胡蓉, 徐如祥. Toll样受体4在小鼠小脑组织中的表达情况分析[J/OL]. 中华神经创伤外科电子杂志, 2018, 04(04): 226-230.

Jianwei Zhu, Yifei Li, Zhaotao Wang, Weiqiang Jia, Chen Chen, Yingjiang Gu, Hu Rong, Ruxiang Xu. Expression of Toll-like receptor 4 in mouse cerebellum[J/OL]. Chinese Journal of Neurotraumatic Surgery(Electronic Edition), 2018, 04(04): 226-230.

目的

分析Toll样受体4(TLR4)蛋白在小鼠小脑组织中的表达情况。

方法

选取6月龄大小的TLR4基因敲除(TLR4-/-)小鼠及其同窝对照的野生型(TLR4+/+)小鼠的小脑组织,制成冰冻切片,通过免疫荧光染色技术检测TLR4在TLR4+/+小鼠小脑冰冻切片组织中的表达,并利用小脑组织特定细胞的蛋白标记Marker与其共染,TLR4-/-小鼠小脑冰冻切片作为阴性对照,最后通过激光共聚焦显微镜获取图像并确定TLR4在小脑组织中的表达。

结果

TLR4高表达于钙结合蛋白阳性的小脑蒲肯野细胞中,少量表达于离子钙接头蛋白阳性的小胶质细胞中,而在性别决定区Y盒2号蛋白阳性的伯格曼胶质细胞和神经元特异核蛋白阳性的颗粒细胞中并无表达。

结论

TLR4高表达于小鼠小脑蒲肯野细胞中,深入分析TLR4在蒲肯野细胞功能执行中的作用,将有助于揭示TLR4在小脑功能执行中的作用。

Objective

To analysis the expression of Toll-like receptor 4 (TLR4) in mouse cerbellum.

Methods

Fronze sections gained from the 6 months old TLR4 knockout (TLR4-/-) mice and wildtype littermates (TLR4+/+). Co-stained TLR4 with cerebellar special cells markers by immunofluorescence technology in frozen sections and revealed TLR4 localization by laser confocal scanning.

Results

TLR4 is highly expressed in Purkinje cell but not in Bergamnn glia or cerbellar granule cells. In addition, there were few microglia expressing TLR4 in cerebellum.

Conclusion

TLR4 is highly expressed in cerebellar Purkinje cell and it is beneficial for revealed its function in cerebellum by analyzing the role of TLR4 on Purkinje cell function.

图1 免疫荧光染色检测Toll样受体4在小脑蒲肯野细胞中的表达
图4 免疫荧光染色检测Toll样受体4在小脑小胶质细胞中的表达
图5 免疫荧光染色检测Toll样受体4在TLR4-/-小脑组织中的表达
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